NIBP在结肠癌NF-κB非经典激活通路中的作用及其临床意义  

Relationship between expression of NIBP and noncanonical NF-κB signaling:Clinical significance in colon carcinoma

作　　者：谭林[1] 刘诗权[1] 覃蒙斌[1] 李素艳[1] 刘宝玉[1] 诸葛春凤 徐春燕[1] 梁梦紫 彭鹏[1] 黄杰安[1] 

机构地区：[1]广西医科大学第一附属医院消化内科,广西壮族自治区南宁市530021

出　　处：《世界华人消化杂志》2015年第8期1238-1246,共9页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;Nos.81260365;81460380;广西卫生厅基金资助项目;No.Z2012103;广西自然科学基金项目;No.2013GXNSFAA019159~~

摘　　要：目的:检测结肠癌患者组织中NIK-IKKβ结合蛋白(NIK,IKKβ-binding protein,NIBP)、磷酸化p100(p-p100)、p52和上皮间质转化(epithelial-mesenchymal transition,EMT)相关通路蛋白:CD44、波形蛋白(Vimentin)和E-钙黏蛋白(E-cadherin)的表达,探讨NIBP蛋白的表达与非经典核因子-κB(nuclear factor-κB,NF-κB)通路的关系及其在结肠癌发生、发展、浸润和转移中的作用.方法:采用免疫组织化学SP法分别检测50例正常结肠黏膜、20例结肠腺瘤以及114例结肠癌患者组织中NIBP、p-p100、p52、CD44、Vimentin和E-cadherin的表达.结果:免疫组织化学结果显示有转移结肠癌组织中NIBP、p-p100、p52、CD44和Vimentin的阳性表达均高于无转移结肠癌以及结肠腺瘤和正常结肠黏膜组织,而E-cadherin则正好相反(P<0.05);且NIBP、p-p100、p52、CD44、Vimentin和E-cadherin在结肠癌组织中的表达与浸润深度、TNM临床分期、淋巴结转移和远处转移有关(P<0.05);结肠癌组织中NIBP与p-p100、p52表达之间,C D44与p52、E-cadherin及Vimentin表达之间均存在明显相关性(P<0.05).结论:NIBP可能通过激活非经典NF-κB信号通路诱导EMT的发生,从而促进结肠癌的发生、发展、浸润和转移.AIM： To investigate the expression of NIK, IKKβ binding protein（NIBP）, phosphorylated p100（ p-p100）, p52, CD44, Vimentin and E-cadherin in colon carcinoma and to explore their clinical significance.METHODS： Immunohistochemistry was used to detect the expression of NIBP, p-p100, p52, CD44, E-cadherin and Vimentin in 114 paraffin-embedded colon carcinoma tissues, 20 colon adenoma tissues and 50 normal colon mucosa tissues.RESULTS： The positive expression of NIBP, p-p100, p52, CD44 and Vimentin in colon cancer tissues with metastasis was much higher than that in colon cancer tissues without metastasis, colon adenoma tissues and normal colon mucosa tissues, while the expression of E-cadherin showed a reverse trend（P〈0.05）. The expression of NIBP, p-p100, p52, CD44, Vimentin and E-cadherin in colon cancer tissues was related with depth of invasion, TNM stage, lymph node metastasis and distant metastasis（P〈0.05）. There were significant correlations between the expression of p-p100 and p52 or NIBP, and between the expression of CD44 and p52, E-cadherin, or Vimentin. CONCLUSION： NIBP may regulate the epithelialmesenchymal transition process by activating thenoncanonical nuclear factor-κB（NF-κB） signal pathway and thus play an important role in the development, invasion and metastasis of colon cancer.

关 键 词：结肠癌 NIK-IKKβ结合蛋白 非经典NF-κB信号通路 浸润 转移 

分 类 号：R735.35[医药卫生—肿瘤]