微小RNA101的表达与结直肠癌患者临床病理特征及预后的关系  

Association of microRNA101 expression with clinicopathologic features and prognosis in colorectal cancer

作　　者：高显华[1] 张卫[1] 袁捷[1] 徐小雯[1] 何建[1] 傅传刚[1] 

机构地区：[1]第二军医大学附属长海医院肛肠外科,上海200433

出　　处：《中华胃肠外科杂志》2015年第4期365-369,共5页Chinese Journal of Gastrointestinal Surgery

基　　金：国家自然科学基金（81201936,81272670,81272561）

摘　　要：目的：探讨微小RNA101（miR-101）在结直肠癌中的表达及其临床意义。方法采用锁定核苷酸原位杂交（LNA-ISH）技术检测组织芯片（56例正常黏膜、51例腺瘤和735例结直肠癌组织）中miR-101的表达水平，并分析其与患者临床病理特征及预后的关系。再用茎环RT-PCR法检测miR-101在5例正常黏膜、5例腺瘤和47例结直肠癌新鲜组织中的表达水平，验证LNA-ISH结果的准确性。结果从正常黏膜、腺瘤到Ⅰ期结直肠癌，miR-101的表达水平逐渐上升；从Ⅱ期、Ⅲ期到Ⅳ期结直肠癌，miR-101的表达水平逐渐下降（P＜0．01）。 miR-101高表达患者的淋巴结转移率低、远处转移率低、分化程度高及复发率低（均P＜0．01）。多因素预后分析显示，miR-101表达可独立预测结直肠癌患者的总生存率（HR=0.550，95% CI：0.351~0.863）和无瘤生存率（HR=0.562，95% CI：0.397~0.794），miR-101高表达提示患者预后较好。结论 miR-101与结直肠癌的发生发展有关，是预后的独立预测因素。Objective To investigate the microRNA101 （miR101） expression and its clinical significance in colorectal cancer. Methods Tissue microarrays containing 56 specimens of normal mucosa, 51 adenoma and 735 colorectal cancer were examined by locked nucleic acid in-situ hybridization （LNA-ISH） for miR101 expression. Relationship between miR101 expression and clinicopathologic parameters and prognosis of colorectal cancer patients were analyzed. Fresh frozen tissues containing 5 specimens of normal mucosa , 5 adenoma and 47 colorectal cancer were examined by RT-PCR to verify the accuracy of LNA-ISH. Results Expression of miR101 increased gradually from normal mucosa, adenoma to stage I colorectal cancer （all P〈0.01）, and decreased gradually from stageⅡ, stage Ⅲ to stage Ⅳ colorectal cancer （all P〈0.01）. Overexpression of miR101 was related with lower incidence of lymph node metastasis, lower metastasis rate, higher differentiation and lower recurrence rate （all P〈0.01）. Multivariate survival analysis demonstrated that miR101 expression was an independent prognostic factor of overall survival （HR=0.550, 95% CI： 0.351-0.863） and disease free survival （HR=0.562, 95% CI： 0.397-0.794） in colorectal cancer patients. Overexpression of miR101 predicted a better prognosis in colorectal cancer patients. Conclusions Expression of miR101 is associated with the genesis and development of colorectal cancer , and may serve as an independent prognostic factor for colorectal cancer patients.

关 键 词：结直肠肿瘤 微小RNA 101 原位杂交 预后 

分 类 号：R735.34[医药卫生—肿瘤]