9种野生牡丹籽油主要脂肪酸成分分析  

Analysis of Fatty Acid in Seed Oil from Nine Wild Peony Species

作　　者：张延龙[1] 韩雪源 牛立新[1] 张静[2] 何丽霞 

机构地区：[1]西北农林科技大学林学院,杨凌712100 [2]西北农林科技大学园艺学院,杨凌712100 [3]甘肃省林业科学技术推广总站,兰州730046

出　　处：《中国粮油学报》2015年第4期72-75,79,共5页Journal of the Chinese Cereals and Oils Association

基　　金：陕西省林业厅项目(陕林计字[2011]70号)

摘　　要：开展我国不同牡丹资源种子的脂肪酸成分评价,对于进一步筛选与培育优良油用牡丹新品种具有重要意义。试验对9种野生牡丹和1个牡丹栽培品种‘凤丹’(对照)的籽油进行提取,使用气质联用色谱法(GC-MS)分析其脂肪酸成分,并采用内标法对其主要脂肪酸进行定量分析。结果表明,不同牡丹种籽的出油率存在差异,杨山牡丹(Paeonia.ostii)的出油率最高,达到22.31%;牡丹籽油中含有丰富的不饱和脂肪酸,依次为亚麻酸、亚油酸和油酸;不同牡丹种的籽油中主要脂肪酸的总含量差异显著,狭叶牡丹(P.potaninii)籽油中主要脂肪酸的总含量最高(90.29 g/100 g粗提油),显著高于对照栽培品种‘凤丹’(P.ostii‘Feng Dan’)籽油中的含量(71.62 g/100 g粗提油);不同牡丹种的籽油中单体脂肪酸的含量差异显著,狭叶牡丹(P.potaninii)籽油中亚麻酸的含量(39.45 g/100 g粗提油)是最低含量四川牡丹(P.decomposita)中的3倍,亚油酸的含量(25.06 g/100 g粗提油)也显著高于最低含量卵叶牡丹(P.qiui)(9.03 g/100 g粗提油)。因此,在油用牡丹新品种选育和种质资源评价方面,要综合考虑不同种和生态型间的差异。Analyzing and evaluating fatty acid compositions of peony seed oil from different peony species are significant for selecting new varieties and oil peony breeding. Supercritical CO2 Extraction has been applied to extract peony seed oil from nine wild species of peonies and one cultivar peony （ as control group）. The fatty acid compositions have been determined by gas chromatography - mass spectrometry （ GC - MS） and quantified by internal standard method. The experimental data have been analyzed through one -way ANOVA. The results indicated that the oil contents were different among different peony species, and oil extraction rate of Paeonia ostii was the highest （ 22.31% ）. Peony seed oil was rich in unsaturated fatty acids, which included linolenic acid, linoleic acid and oleic acid. There were significant differences in the total contents of fatty acids among different species P. potaninii showed the highest content of total fatty acids （90.29 g/100 g crude oil） which were significantly higher than that of the control P. ostii＇ Feng Dan＇. There were also obvious differences in the contents of monomer fatty acid among different species. Linolenic acid content of P. potaninii （39.45 g/100 g crude oil）was almost three times of that of P. decomposita, and linoleic acid content of P. ostii ＇Feng Dan＇ （25.06 g/100 g crude oil） was also distinctly higher than that of P. qiui （9.03 g/100 g crude oil）. In conclusion, for the selection of new varieties and germplasm resources evaluation of tree peony, the differences among species and ecotypes should be taken into consideration.

关 键 词：牡丹籽油 内标法 脂肪酸 定量分析 

分 类 号：TQ646.4[化学工程—精细化工]