食品中亚硝酸盐快速检测试纸条的研制  

Development of Test Strips for Rapid Detection of Nitrites in Food

作　　者：贾宇旻 吴晓婵[1] 渠光辉 黎文娟[1] 李晓云[2] 

机构地区：[1]湖南农业大学东方科技学院,湖南长沙410128 [2]湖南农业大学动物医学院,湖南长沙410128

出　　处：《中国动物检疫》2015年第4期72-74,共3页China Animal Health Inspection

基　　金：2013年湖南农业大学东方科技学院大学生创新性实验计划(DFCXY201301);2009年湖南农业大学动物医学院大学生创新性实验计划"腌腊制品中发色剂与防腐剂快速检测"

摘　　要：利用与对氨基苯磺酸和盐酸萘乙二胺反应显色的原理,探索最佳的试纸卡制作工艺,将对氨基苯磺酸和盐酸萘乙二胺分别浸在试纸上,干燥,组装成亚硝酸盐速测试纸卡。同时制备含量依次为100 mg/L、80 mg/L、60 mg/L、40 mg/L、20 mg/L、10 mg/L、5.0mg/L、2mg/L、1mg/L的亚硝酸盐溶液制成标准比色卡,作为比色对照。经与分光光度法做了灵敏度和准确度的对比,该方法具有快速、简便、灵敏度和准确度高等特点。The best process for preparation of test strips for rapid detection of nitritesin food was explored by using the color reaction principle of sulphanilic acid and naphthyl ethylenediamine dihydrochloride. The sulphanilic acid and naphthyl ethylenediamine dihydrochloride were respectively soaked on to the test paper and the test paper was assembled to rapid test strips when dried. Meanwhile,reference colorimetric card was prepared with 100 mg/L,80 mg/L,60 mg/L,40 mg/L,20 mg/L,10 mg/L,5.0mg/L 2mg/L,1mg/L nitrites as colorimetric control. The method was rapid,simple,sensitive and accurate as compared with spectrophotometry.

关 键 词：亚硝酸盐 快速检测 检测试纸条 分光光度法 

分 类 号：TS207[轻工技术与工程—食品科学]