RNA干扰下调宫颈癌Hela细胞Survivin基因对Hela细胞增殖能力影响的研究  

Effects of the down- regulated expression of Survivin gene by RNA interference on proliferation ability of cervical carcinoma cell Hela

作　　者：李元宏[1] 李卫萍[1] 

机构地区：[1]山西医科大学汾阳学院,山西汾阳032200

出　　处：《临床医药实践》2015年第4期248-251,共4页Proceeding of Clinical Medicine

摘　　要：目的:通过RNA干扰技术下调宫颈癌Hela细胞Survivin基因表达,观察Hela细胞增殖能力的变化情况。方法:当细胞生长至70.0%左右覆盖率时进行脂质体转染,细胞分为空白组(Hela细胞未转染组)、阴性对照组(转染空质粒)、实验组[Survivin-siRNA(+)质粒转染Hela细胞]三组。采用脂质体介导的方法将特异针对Hela细胞Survivin基因的干扰RNA转染入细胞后不同时间观察细胞生长形态的变化,记录转染后第1天到第5天每天的生长情况并绘制生长曲线,通过四甲基偶氮唑蓝(MTT)实验记录24 h,48 h,72 h活细胞数量,总结分析细胞生长情况。结果:RNA干扰后出现细胞生长速度减慢,实验组与空白组比较,从第3天到第5天空白组细胞数明显高于实验组(第3天P<0.05,第4天、第5天P<0.01)。实验表明,干扰24 h后实验组活细胞数量与空白组、阴性对照组比较,差异均无统计学意义(P>0.05),48 h及72 h实验组活细胞数明显低于空白组与阴性对照组(P<0.01)。结论:Survivin基因沉默能够有效特异地抑制宫颈癌Hela细胞的体外恶性增殖能力,有利于细胞的凋亡,Survivin基因可以作为宫颈癌基因治疗的靶点。Objective：The expression of survivin gene was regulated down by the technology of RNA interference in cervi-cal cancer to observe the changes of Hela cell proliferation ability. Methods：Transfected by liposome when the cells were grown to about 70. 0% coverage,the cells were divided into 3 groups,which were blank group（non—transfection group Hela cells）, negative control group（ empty plasmid transfection group）and the experimental group（ survivin—siRNA（ ＋）plasmid trans-fection group ）. After interfering RNA specific for survivin gene of the cell Hela was transfected into Hela cells by the method of liposome induction,the morphological changes of cell growth were observed at different times,simultaneously the growth con-ditions of the cell growth were recorded respectively from 1d p. i. to 5 d p. i. ,then the growth curve were drew up accordingly. Recorded the number of living cells at 24 h,48 h,72 h by MTT assay and analyzed the conditions of cell growth. Results：By comparising the experimental group where the growth rate slows down by RNA Interference and the blank group,the number of the blank group cells was significantly higher than of the experimental group 3 d p. i. to 5 d p. i.（3 d p. i. P0. 05）,however,viable count of the experimental group was significantly lower than of the control group and negative control group at 48h p. i and 72h p. i.（P〈0. 01）. Conclusion：survivin gene silencing could inhibit the malignant proliferation ability of cervical carcinoma cell Hela in vitro,meanwhile,favor cell apoptosis. The survivin may serve as the target for cervical cancer by gene therapy.

关 键 词：HELA细胞 SURVIVIN 细胞增殖 

分 类 号：R737.33[医药卫生—肿瘤]