沉默信息调节因子1在膀胱尿路上皮细胞癌中的表达与意义  

Expression and clinical significance of silent information regulator 1 in bladder urothelial cell carcinoma

作　　者：蓝宇[1] 刘东东[1] 林明恩[1] 洪英楷[1] 黄建生[1] 陈思[1] 何学军[1] 

机构地区：[1]汕头大学医学院第一附属医院泌尿外科,广东汕头515041

出　　处：《癌变．畸变．突变》2015年第2期125-128,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:检测沉默信息调节因子1(SIRT1)在膀胱尿路上皮癌(BUC)中的表达,并探讨其与临床病理指标的相关性。方法:选取经临床病理诊断的BUC标本72例,其中低级别尿路上皮癌42例,高级别尿路上皮癌30例;阴性对照组为正常膀胱组织19例(膀胱镜检术或膀胱切开取石术中随机取活检时取得)。应用免疫组化方法(二步法)检测SIRT1分别在低级别与高级别BUC中的表达与分布,分析其与肿瘤的分期和病理分级的关系。结果:SIRT1在正常膀胱组织和膀胱癌组织中均有表达,正常膀胱组织中阳性表达率为31.58%(6/19),而在BUC组中,阳性表达率为77.78%(56/72),明显高于正常膀胱组织(P<0.05);并且随着肿瘤分期和癌理分级的升高SIRT1蛋白的表达增加,其中低级别膀胱癌阳性表达率为69.05%(29/42),高级别膀胱癌阳性表达率为90.00%(27/30),两者间的差异具有统计学意义(P<0.05)。结论:SIRT1在BUC中高表达,可能与BUC的发生、病理分级和临床分期有关,并有可能作为判断BUC侵袭力、监测复发的肿瘤标志物。阻断SIRT1表达有望成为膀胱癌靶向治疗的新靶点。OBJECTIVE： To investigate the expression of silent information regulator I（SIRT1） in bladder urothelial cell carcinoma （BUC）, and analyze its clinical significance. METHODS ： The expression of SIRT1 protein was assessed by immunohistochemistry in bladder sections of 19 normal bladders and 72 patients with BUC. We analyzed the relationship of SIRT1 and the pathological grade and clinical stage of BUC. RUSULTS： SIRT1 was observed in normal bladder samples and BUC. Normal bladder showed negative or weakly positive expression of SIRT1（31.58%）, however BUC samples showed moderately or strongly positive expression（77.78%）. Furthermore, the expression level of SIRT1 increased with tumor pathological grade and clinical stage. SIRT1 positive expression rates showed significant difference between various pathology grades（P〈0.05）. CONCLUSION： SIRT1 was high expressed in BUC, and was related to its pathological grade and clinical stage, making it a useful tumor marker and a possible indicator for estimating the invasiveness and recurrence of BUC. Blocking SIRT1 expression and its functions may become targeted therapy of bladder cancer treatment.

关 键 词：膀胱尿路上皮癌 沉默信息调节因子1 免疫组化 肿瘤标志物 

分 类 号：R737.1[医药卫生—肿瘤]