nm23-H1基因转染对肺癌L9981细胞黏附分子表达的影响  

Influences of nm23-H1 gene transfection on expressions of adhesion molecules in lunq cancer cell line L9981

作　　者：郑海霞[1] 崔永言[2] 申东兰[3] 彭安[3] 何艳玲[3] 

机构地区：[1]北京大学深圳医院体检科,广东深圳518036 [2]北京大学深圳医院整形外科,广东深圳518036 [3]北京大学深圳医院肿瘤内科,广东深圳518036

出　　处：《癌变．畸变．突变》2015年第2期134-136,141,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金资助项目(30070333)

摘　　要：目的:探讨nm23-H1基因转染对肺癌L9981细胞黏附分子表达的影响。方法:利用细胞黏附实验、Boyden小室法研究转染前后肺癌L9981细胞黏附、侵袭能力的改变。分别利用逆转录-PCR(RT-PCR)和流式细胞术检测转染前后E-cadherin、integrinβ1和integrinβ3 mRNA和蛋白的表达。结果:转染后L9981细胞株黏附性以及侵袭性均降低。与转染空载体组相比,转染nm23-H1质粒后L9981细胞E-cadherin mRNA和蛋白表达均增强,integrinβ1和integrinβ3 m RNA和蛋白表达均减弱,差异均具有统计学意义(P均<0.05)。结论:nm23-H1基因具有逆转肺癌恶性转移表型的能力,其对肺癌L9981细胞株E-cadherin表达具有正调控作用,对integrinβ1和integrinβ3表达具有负调控作用。OBJECTIVE： To study influences molecules in lung cancer cell line L9981. METHODS： of nm23-H1 gene transfection on the expressions of adhesion Cell adhesion experiment and Boyden room were used to evaluate the change of cell adhesion and invasion. Semi-RT-PCR and flow cytometry were used to detect the mRNA and protein expressions of E-cadherin, integrin β1 and integrin β3 betwteen transfected and non-transfected cell lines. RESULTS： nm23-H1 cell line in vitro adhesion and invasion were all down-regulated, mRNA of E-cadherin in the transfected cell line was stronger than that of non-transfeeted cell line, but mRNAs of integrin β1 and integrin β3 of transfected cell line were weaker than that of non-transfected cell line. Flow eytometry showed similar results as above. CONCLUSION： nm23-H1 could reverse lung cancer malignant phenotype, nm23-H1 could up-regulate E-eadherin expression, while down-regulate expressions of integrins β1 and β3, thus inhibit the metastasis of lung cancer cells.

关 键 词：NM23-H1 肺癌细胞 钙调素 整合素 肿瘤转移 

分 类 号：R730.2[医药卫生—肿瘤]