XB130对胶质瘤细胞株LN229增殖和凋亡的影响  

Influence of XB130 on proliferation and apoptosis of glioma cell line LN229

作　　者：孙树鹏[1] 王琼[2] 王修玉[3] 王金环[2] 

机构地区：[1]天津市环湖医院 [2]天津市环湖医院,天津市脑血管与神经变性重点实验室 [3]天津医科大学研究生院,300070

出　　处：《中华神经外科杂志》2015年第4期376-380,共5页Chinese Journal of Neurosurgery

基　　金：天津市科委抗癌重大专项攻关计划项目（12ZCDZSY17700）;天津市科委重点项目（14JCZDJC35600）;天津市卫生局科技攻关项目（11KGl15）

摘　　要：目的 探讨下调胶质瘤细胞株LN229中XB130的表达对细胞增殖和凋亡的影响.方法 选取LN229细胞作为研究对象,分为对照组、无义序列siRNA转染组和XB130 siRNA转染组进行细胞培养,细胞转染siRNA后,采用Western blot和免疫荧光检测XB130的表达情况;用MTT检测细胞的增殖活性;流式细胞仪检测细胞凋亡及周期.结果 转染siRNA后,对照组、无义序列siRNA转染组、XB130 siRNA转染组XB130蛋白相对表达量分别为1.00 ±0.10、1.00±0.13、0.52±0.06,XB130平均免疫荧光强度分别为0.085±0.012、0.072±0.009、0.013±0.002,表明XB130siRNA转染组XB130蛋白表达显著降低,差异有统计学意义（P＜0.05）;MTr结果表明XB130 siRNA转染组细胞增殖受到明显抑制,差异有统计学意义（P＜0.05）;流式细胞仪检测发现对照组、无义序列siRNA转染组、XB130 siRNA转染组细胞凋亡率分别为（12.45±0.96）％、（13.64±0.85）％、（20.26±1.42）％,G0/G1期细胞比例分别为（62.85±1.85）％、（63.04±1.74）％、（72.67±1.93）％,表明XB130 siRNA转染组细胞凋亡率显著高于其他两组,差异有统计学意义（P＜0.05）,G0/G1期细胞比例也显著高于其他两组,差异有统计学意义（P＜0.05）.结论 沉默胶质瘤细胞中XB130的表达能显著抑制细胞的增殖并促进其凋亡,表明XB130蛋白可促进胶质瘤细胞的增殖。Objective To investigate the influence of XB130 on the proliferation and apoptosis of glioma cell LN229.Methods LN229 cells were divided into the control group,nonsense sequence siRNA transfected group and XB130 siRNA transfected group.After siRNAs were transfected into LN229 cells,the expression of XB130 was detected by Western blot and immunofluorescence staining.The phenotypic changes of LN229 cells including proliferation,apoptosis and cell cycle were determined by MTT assay and flow cytometry.Results The relative protein expression levels and immunofluorescence intensity of XB130 were respectively 1.00 ± 0.10 and 0.085 ± 0.012 in the control group,1.00 ± 0.13 and 0.072 ± 0.009 in the nonsense sequence group,0.52 ± 0.06 and 0.013 ± 0.002 in the XB130 siRNA transfected group.The expression of XB130 in LN229 cells was significantly decreased in the XB130 siRNA transfected group （P 〈 0.05）.LN229 cells transfected with XB130 siRNA showed lowering proliferation activity by MTT assay （P 〈 0.05）.The results of flow cytometry showed that the apoptotic rate and the proportion of cells in G0/G1 phase were （12.45 ± 0.96） % and （62.85 ± 1.85） % in the control group,（13.64 ± 0.85） % and （63.04 ± 1.74） % in the nonsense sequence group,（20.26 ± 1.42） % and （72.67 ± 1.93） % in the XB130 siRNA transfected group respectively.The apoptotic rate and the proportion of cells in G0/G1 phase in the XB130 siRNA transfected group were significantly higher than those in the other two groups （P 〈0.05）.Conclusions Knocking down the expression of XB130 in human glioma cells could significantly inhibit cell proliferation and induce apoptosis,indicating XB130 could promote the proliferation of glioma cells.

关 键 词：信号传导衔接蛋白 神经胶质瘤 细胞增殖 细胞凋亡 

分 类 号：R739.41[医药卫生—肿瘤]