^(99)Tc^m-ZPPAb在荷H_(22)肝癌小鼠体内的生物学分布特性及显像特征  

Biodistribution and imaging features of ^(99)Tc^m labelling ^(99)Tc^m-ZPPAb in bearing H_(22) hepatocarcinoma mice in vivo

作　　者：郑立春[1] 冯珏[2] 张晓明[1] 张文军[1] 

机构地区：[1]唐山市工人医院核医学科,河北唐山063000 [2]河北医科大学第二医院核医学科,河北石家庄050000

出　　处：《中国医学影像技术》2015年第4期498-502,共5页Chinese Journal of Medical Imaging Technology

摘　　要：目的探讨99 Tcm-ZPPAb在荷瘤鼠体内生物学分布特征及显像特征。方法 1建立荷H22肝癌小鼠模型48只,分为实验组及对照组,各24只。制备99 Tcm-ZPPAb及99 Tcm-IgG。2体内分布实验:取实验组与对照组各21只小鼠,分别经尾静脉注射99 Tcm-ZPPAb和99 Tcm-IgG 7.4MBq,于30min、1、2、3、4、5、6h,每时相取3只,计算各脏器每克组织的放射性摄取比率(%ID/g值)及肿瘤与对侧肌肉组织的放射性计数比值(T/NT值)。3显像实验:取实验组与对照组各3只小鼠,分别经尾静脉注射99 Tcm-ZPPAb和99 Tcm-IgG 7.4MBq,于30min、1、2、3、4、5、6h计算T/NT值。结果 99 Tcm ZPPAb标记率为70%,99 Tcm-IgG标记率为85%。体内分布实验结果显示,实验组荷瘤小鼠体内肿瘤组织不同时相间%ID/g值差异无统计学意义(F=0.89,P=0.5231),各时相肿瘤组织%ID/g值均高于除肝脏、肾脏和血液以外的其他组织器官(F=9.36,P=0.0007)。两组间同时相肿瘤组织%ID/g值差异有统计学意义(F=13.49,P=0.0001)。实验组不同时相T/NT值差异无统计学意义(F=1.53,P=0.2237);两组间同时相T/NT值差异有统计学意义(F=23.69,P=0.0001)。显像实验结果显示,实验组不同时相T/NT值差异无统计学意义(F=1.00,P=0.4526),两组间同时相T/NT值差异有统计学意义(F=6.09,P=0.0048)。结论 99 Tcm-ZPPAb标记简单,标记率较高,在肿瘤组织中有较高的摄取与滞留,有望成为肿瘤阳性显像剂。Objective To investigate the biodistribution and imaging features of 99Tcm-ZPPAb in bearing H22 hepatocarcinoma mice in vivo.Methods 1A total of 48 mice bearing H22 hepatocarcinoma were divided into study group and control group with 24 mice in each group.Labeling ZPPAb and IgG with 99Tcm were prepared.2Biodistribution experiment：21mice in each group were separately injected 99Tcm-ZPPAb for study group and 99Tcm-IgG 7.4MBq for control group through the tail intravenous.The value of%ID/g of each tissue and T/NT of the tumor to contralateral muscle were calculated in 3mice of each group at 30 minutes,1,2,3,4,5,6hours respectively.3Imaging experiment：Totally 6 mice in study group（n=3）and control group（n=3）were injected 99Tcm-ZPPAb and 99 Tcm-IgG 7.4MBq through the tail intravenous,image at 30 minutes,1,2,3,4,5,6hours.And the value of T/NT was calculated.Results The labeling rate of 99TcmZPPAb was 70%and that of 99 Tcm-IgG was 85%.Biodistribution experiment：There was no significant difference of%ID/g among different phase（F=0.89,P=0.5231）.The%ID/g of tumor in each phase was respectively higher than that of other tissues except of liver,kidney and blood（F=9.36,P=0.0007）.In the same phase,significant difference of tumor%ID/g was found between the two groups（F=13.49,P=0.0001）.No significant difference of T/NT was found among different phase in study group（F=1.53,P=0.2237）.In the same phase,there was significant difference of T/NT between the two groups（F=23.69,P=0.0001）.Imaging experiment：There was no significant difference of T/NT was found among different phase in study group（F=1.00,P=0.4526）.In the same phase,significant difference of T/NT was found between the two groups（F=6.09,P=0.0048）.Conclusion 99 Tcm-ZPPAb can be conveniently labeled with satisfactory labelling efficiency.And it can be well uptaked and retented in tumor tissue.99Tcm-ZPPAb will be a good positive tumor imaging agent.

关 键 词：郑氏植物蛋白 小鼠 99M锝美罗酸盐 生物分布 放射性核素显像 

分 类 号：R-332[医药卫生] R817