鞣料云实素的体外抗炎作用  

Anti-inflammatory Role of Corilagin in vitro

作　　者：谢函君 李刚[2] 

机构地区：[1]湖北省仙桃市中医医院,湖北仙桃433000 [2]湖北医药学院附属人民医院感染性疾病科,湖北十堰442000

出　　处：《武汉大学学报（医学版）》2015年第3期341-343,共3页Medical Journal of Wuhan University

摘　　要：目的:初步探讨鞣料云实素在细胞模型上的抗炎机制。方法:采用脂多糖(LPS)刺激的小鼠巨噬细胞瘤细胞RAW 264.7建立炎症模型,同时用鞣料云实素进行干预,ELISA法检测细胞上清中TNF-α,IL-1β,IL-6的含量,Real-time PCR法检测细胞中TNF-α,COX-2mRNA的表达情况。结果:鞣料云实素可以通过在基因和蛋白水平上下调促炎因子TNF-α,IL-1β,IL-6的表达。结论:鞣料云实素对促炎细胞因子和炎性介质TNF-α,IL-1β,IL-6,COX-2均有不同程度的抑制作用,说明鞣料云实素对炎症的起始阶段有明显的抑制作用。Objective：To investigate the anti-inflammatory mechanism of Corilagin on cellular model.Methods：The cellular model was set up with RAW264.7cells simulated by LPS.Levels of TNF-α,IL-1β,and IL-6in supernatant were tested by ELISA.Real-time PCR was employed to detect the TNF-αand COX-2mRNA levels.Results：Corilagin could suppress the expression of TNF-α,IL-1βand IL-6both on mRNA and protein levels.Conclusion：Corilagin has an effect on initial stage of inflammation process via inhibiting the expression of pro-inflammatory cytokines TNF-α,IL-1β,IL-6and COX-2.

关 键 词：鞣料云实素 RAW264.7细胞 炎症反应 促炎因子 

分 类 号：R-28[医药卫生]