全脑缺血再灌注后海马EphA受体基因表达的变化特点  

Gene Expression of Eph A Receptors in Hippocampus of Rats after Transient Global Ischemia

作　　者：杨锦珊[1,2] 龙根 徐莉[1] 谢敏杰[1] 王伟[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030 [2]福建医科大学附属第一医院神经内科,福州350000

出　　处：《神经损伤与功能重建》2015年第2期102-106,共5页Neural Injury and Functional Reconstruction

基　　金：国家自然科学基金重点项目(No.81030021)

摘　　要：目的:观察EphA受体在海马全脑缺血再灌注后基因表达的改变。方法:SD大鼠50只随机分为假手术组10只及全脑缺血再灌注组40只,Pulsinelli四血管阻断法建立全脑缺血再灌注模型,采用半定量RT-PCR观察假手术组及缺血组在缺血后不同时间点(6 h、1 d、3 d、7d)EphA受体m RNA含量变化的情况,免疫荧光双标法检测EphA4受体在海马的细胞定位。结果:EphA1-A8及EphA10 RNA在正常海马组织均有表达,EphA4受体含量多。在缺血状态下,EphA1、EphA2、EphA3、EphA6、EphA7及EphA8的mRNA表达水平一过性上调,EphA4、EphA5和EphA10的m RNA表达水平逐步上调;EphA4受体亦是缺血后变化最显著的EphA受体。免疫荧光双标显示EphA4主要分布于海马CA1-CA3区及DG区Neu N阳性锥体神经元。结论:在海马不同的EphA受体对缺血呈现出不同的应答模式,EphA4是海马正常条件下表达最为丰富的EphA受体并且在缺血条件下出现最为显著地表达变化。EphA4受体主要分布于海马CA1-CA3区以及DG区NeuN阳性锥体神经元。Objective： To observe the gene expression of EphA receptors in the normal and ischemic hippocampus in rats. Methods： Fifty SD rats were randomly divided into two groups： sham group（n=10） and ischemia group（n=40）. The Pulsinelli four-vessel occlusion model was used to induce transient global ischemia. Semi-quantitative RT-PCR was applied to measure gene expressions of EphA receptors in hippocampus at different time points（6h,1d, 3d, 7d）. To detect the cellular location of EphA4 receptors in the hippocampus,dual immunofluorescence was employed. Results： All EphA receptors including EphA1-A8 and EphA10 were expressed in normal hippocampus,and EphA4 the most abundantly expressed in normal hippocampus. EphA receptors exhibited different change patterns of RNA expressions in the hippocampus after transient global ischemia. EphA1, EphA2, EphA3, EphA6,EphA7 and EphA8 presented a transient up-regulation of RNA expression. However, EphA4, EphA5 and EphA10 expressed in persisted up-regulation of RNA since transient global ischemia. EphA4 also the most significantly expressed in the ischemic hippocampus. Immunofluorescence study showed that EphA4 was mainly distributed in the Neu N-positive pyramidal neurons in the hippocampus, especially in the CA1-CA3 and DG regions. Conclusion：EphA receptors exhibited different patterns in RNA expression in the hippocampus after ischemia. EphA4 receptor,the most abundantly expressed in normal hippocampus, experienced dramatic change in the ischemic hippocampus.EphA4 was mainly located in the NeuN-positive pyramidal neurons in the hippocampus, especially in the CA1-CA3 and DG regions.

关 键 词：EphA受体 短暂性全脑缺血模型 海马 EphA4受体 锥体神经元 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.02[医药卫生—临床医学]