可注射牙槽骨修复材料的软组织反应评价  

In vivo soft tissue response to an injectable alveolar bone substitute

作　　者：张智星[1] 冯祥礼[2] 毛靖[1] 肖建中[3] 邱进俊[4] 刘承美[4] 

机构地区：[1]华中科技大学同济医学院附属同济医院口腔医学中心,湖北武汉430030 [2]湖北省中医院口腔科,湖北武汉430061 [3]华中科技大学材料科学与工程学院,湖北武汉430079 [4]华中科技大学化学学院,湖北武汉430079

出　　处：《临床口腔医学杂志》2015年第4期199-203,共5页Journal of Clinical Stomatology

基　　金：国家高技术研究发展计划(863计划)资助项目(2006AA03Z0443)

摘　　要：目的：研究自制的可注射牙槽骨修复材料植入兔体内时的软组织反应，评价其体内的生物相容性。方法：选用25只新西兰大白兔，按2、4、8、12、24周的植入期分为5组，将β-磷酸三钙（β-TCP）、不饱和聚磷酸酯聚合物（UPPE）、不饱和聚磷酸酯／β-磷酸三钙复合物（UPPE／β-TCP）和添加1％四环素的UPPE／β-TCP复合物（UPPE／β-TCP／TTC）分别植入其皮下，对软组织样本进行组织病理学和组织形态学评价，并进行统计学分析。结果：各组植入体周围的纤维组织囊中均未见成骨细胞、骨细胞或骨组织；术后2周时除UPPE／β-TCP／TIC植入体外，其余植入体均有轻度的炎性细胞浸润；术后2～8周时各组纤维组织囊的厚度均随植入期的延长而增加，在8周时最厚，以后无明显改变；植入体中仅UPPE聚合物在第8周时开始出现降解，纤维组织向材料内部浸润生长；组织形态学分析表明8周时β-TCP、UPPE聚合物和UPPE／β-TCP复合物组的软组织评级得分均低于第2周，组间差异具有统计学意义（P〈0．05）；各时期UPPE／β-TCP／TTC复合物组软组织评级得分的差别无统计学意义（P〉0．05）。结论：可注射牙槽骨修复材料UPPE／β-TCP复合物在动物体内具有良好的软组织生物相容性，添加四环素后有利于减轻UPPE／β-TCP复合物植入早期的炎症反应。Objective： To assess in vivo soft tissue response to the injectable alveolar bone substitute after implanta- tion using a rabbit model. Method： Twenty-five male New Zealand White rabbits were used as experimental animals. For the insertion of the subcutaneous implants,fours subcutaneous pockets at the dorsum of the rabbits were created. The implants （β-TCP, UPPE polymer, UPPE / β-TCP composite and UPPE / β-TCP / TTC composite） were inserted in these pockets for 2,4, 8, 12 and 24 weeks. The specimens were evaluated morphologically （histology and histomorphometry）. Single factor analysis of variance and Tukey＇s HSD multiple comparison tests were used to determine statistical significance of results. Result： The histologic evaluation in vivo after 2 weeks showed a mild infiltrate of inflammatory cells for most of the groups. UPPE / β-TCP / TTC composite exhibited no signal of inflammatory response. After 4 weeks, the tissue showed near normal pattern for all the groups. The soft tissue capsule had a non-uniform distribution in thickness,which increased most signifi- cantly between 4 weeks and 8 weeks after implantation. After 8 weeks, the capsule thickness did not change much. None of polymorphic cells, osteoblast cells or bone cells adjacent to the implant were observed. The surface of the β-TCP, UPPE / β- TCP composite and UPPE / β-TCP / TTC composite themselves remained substantially intact without noticeable cracking, chipping or dissolution. However, at 8 weeks, UPPE polymer showed extensive surface erosion and superficial fragmentation that was surrounded by a few inflammatory cells. Conclusion： UPPE / β-TCP composite is bioeompatible. TTC addition op- timized biocompatibility of the UPPE/β-TCP composite, contributing to anti-inflammatory response during the early phases of the wound healing process.

关 键 词：不饱和聚磷酸酯 Β-磷酸三钙 四环素 软组织反应 

分 类 号：R782.13[医药卫生—口腔医学]