吸烟对口腔黏膜上皮细胞NOD1信号通路关键分子表达水平的影响  

Effects of cigarette smoke on key molecules of NOD1 signaling in human oral mucosal epithelial cells

作　　者：高雅凡 王文梅[1] 王翔[1] 叶沛[1] 钱雅洁[1] 黎景景 

机构地区：[1]南京市口腔医院.南京大学医学院附属口腔医院,江苏南京210008

出　　处：《临床口腔医学杂志》2015年第4期206-209,共4页Journal of Clinical Stomatology

基　　金：国家自然科学基金项目(81070839);江苏省临床医学科技专项(BL2014018);南京市科技计划项目(201402033)

摘　　要：目的:探讨吸烟与口腔黏膜上皮细胞NOD1信号通路关键分子表达水平的关系。方法:采用商业化可购的直接烟暴露装置,构建体外Leuk-1细胞吸烟模型,将其分为吸烟组(分别吸入香烟烟雾5、10、20、40、60 min)和对照组(吸入含5%CO2空气10 min),应用间接免疫荧光法检测体外吸烟对口腔黏膜上皮细胞NOD1通路内关键蛋白的表达。结果:随着体外吸烟时间的延长,NOD1蛋白表达水平逐渐下降,Caspase12和RIP2蛋白表达水平逐渐上升。NF-κB的蛋白表达水平逐渐上升之后明显下降。结论:吸烟可使口腔黏膜细胞NOD1信号通路相关蛋白表达发生改变。Objective： To evaluate the effect of Cigarette Smoke on Key Molecules of NOD1 Signaling in human oral mucosal epithelial cells. Method： The cells were stimulated using a smoke chamber with cigarette smoke for different times （5,10,20,40,60 min） in certain experiments. The control group cells were cultured with 5 % CO2 for 10 min. The crucial protein expression in NOD1 Signaling was measured by Immunofluorescence assays. Result： Cigarette Smoke down-regu- lated NOD1 protein levels and up-regulated Caspase 12 and RIP2 protein in a time-dependent manner. The NF-KB protein level reached the peak level after stimulated for 10 mins. Conclusion： Cigarette smoke could play a inhibitory effects on NOD1 Signaling even innate immunity in human oral mucosal epithelial cells.

关 键 词：NOD1 吸烟 固有免疫 

分 类 号：R781.5[医药卫生—口腔医学] Q786[医药卫生—临床医学]