煅烧骨复合PRF对牙周骨缺损的实验研究  

Empirical Study of Reparation of Periodontal Bone Defects Treated with PRP and Calcined Bone Composite.

作　　者：董红宾[1] 张琴[1] 何惠宇[1] 克依木.克里木 吕晶晶[1] 

机构地区：[1]新疆医科大学第一附属医院口腔修复科,新疆乌鲁木齐830054

出　　处：《口腔医学研究》2015年第4期332-335,339,共5页Journal of Oral Science Research

基　　金：新疆维吾尔自治区科技厅科技支疆项目(编号:201291173);新疆医科大学第一附属医院青年基金项目(编号:2012QN21)

摘　　要：目的:研究比较异种煅烧骨和Bio-oss骨分别与富血小板纤维蛋白(platdet-rich fibrin,PRF)联合,及其各自单独使用对牙周骨缺损修复的效果。方法:制备煅烧骨,PRF凝胶及其复合物。选54只新西兰大白兔,制备牙周组织缺损模型,随机分成空白对照组、PRF凝胶组、煅烧骨组、Bio-oss骨组、煅烧骨/PRF凝胶组、Bio-oss骨/PRF凝胶组,将这些材料植入牙周缺损区,每组9只兔子。术后4周、8周和l2周处死动物,获取完整标本,观察影像学和组织学。行组织学染色检测,测量新生牙周组织的高度。结果:X线结果表明:4周、8周和l2周煅烧骨/PRF组和Bio-oss/PRF组平均密度均明显高于对照组且缺损处骨组织连续。组织学观察:实验组均在牙根面可见新生牙骨质样组织、牙周膜和牙槽骨样组织的形成,随着时间的推移逐渐成熟、增多。12周时,煅烧骨/PRF凝胶、Bio-oss骨/PRF凝胶较空白对照组明显提高,较其他3组差异有统计学意义(P<0.05)。结论:煅烧骨/PRF凝胶、Bio-oss骨/PRF凝胶复合物可明显促进牙周组织的再生。Objective：To study the dissimilar calcined bone and Bio-oss bone respectively,and composite with platelet rich fibrin（platdet-rich fibrin,PRF）,and their respective alone on the effect of periodontal bone defects.Methods：Calcined bone,PRF gel and complex were prepared.54 rabbits were selected.Periodontal tissue defect model was randomly divided into control group,PRF gel group,calcined bone group,Bio-oss bone group,calcined bone/PRF group,Bio-oss bone/PRF group.After four weeks,eight weeks and l2 weeks the animals were sacrificed for a complete specimen,radiological and histological observation.Histological staining was used to measure the height of newborn periodontal tissues.Results：X-ray results showed that：4weeks,8weeks and l2 weeks calcined bone/PRF group and Bio-oss/PRF group were significantly higher than the average density,and bone tissue defects continuously.The experimental group was seen in root surfaces like tissue formation of new cementum,periodontal ligament and alveolar bone-like tissue matures over time,increasing.12 weeks,the calcined bone/PRF,Bio-oss bone/PRF significantly improved compared with the control group,compared with the other three groups were statistically significant（P〈0.05）.Conclusion：Calcined bone/PRF gel and Bio-oss bone/PRF gel complexes can significantly promote the regeneration of periodontal tissues.

关 键 词：富血小板纤维蛋白 煅烧骨 牙周组织再生 

分 类 号：R781.4[医药卫生—口腔医学]