RGD多肽修饰的无机小牛骨粉对MC3T3-E1细胞黏附、增殖及分化的影响  

Adhesion,Proliferation and Differentiation of MC3T3-E1 Cell on Bovine Bone Substitute Modified with RGD Peptide.

作　　者：高媛媛[1,2] 周子谦 柳慧芬 童昕[1,2] 

机构地区：[1]南京大学医学院附属口腔医院 [2]南京市口腔医院种植科,江苏南京210000

出　　处：《口腔医学研究》2015年第4期354-358,共5页Journal of Oral Science Research

摘　　要：目的:研究小鼠前成骨细胞MC3T3-E1在RGD修饰的无机小牛骨粉上的黏附、增殖及分化。方法:浸泡法制备RGD/Bio-Oss复合材料。MTT法检测细胞1h、2h、3h黏附,扫描电镜观察细胞黏附形态,DNA浓度定量分析细胞1d、3d、5d、7d的增殖。碱性磷酸酶(ALP)试剂盒检测细胞7d、14d的分化。结果:1h、2h、3h细胞黏附结果 Bio-Oss/RGD组明显高于Bio-Oss组及空白对照组;1d、3d、5d、7dBio-Oss/RGD组和Bio-Oss组的DNA浓度差别无统计学意义,但都低于空白对照组;细胞分化第14天时Bio-Oss/RGD组的结果明显高于Bio-Oss组与空白对照组。结论:RGD对细胞黏附及分化有一定的促进作用,但对细胞增殖无显著促进作用。Objective：To evaluate the effect of adhesion,proliferation and differentiation of MC3T3-E1 cells on the bovine bone modified by RGD peptide.Methods：In RGD/Bio-Oss group,Bio-Oss was coated with RGD using only deep and dry methods.The MC3T3-E1 cells were seeded on the RGD modified Bio-Oss,after 1h,2h and 3h,the adhesion of MC3T3-E1 cells was determined by MTT assay.The morphology of MC3T3-E1 cells was observed by SEM.The proliferation of MC3T3-E1 cells was determined by DNA concentration after 1d,3d,5dand 7d.The MC3T-E1 cells were cultured on RGD modified Bio-Oss for 7dand 14 dwith osteogenic supplements,the ALP activity of the MC3T3-E1 cells was measured.Results：The cell adhesion of the RGD modified Bio-Oss group was much higher than the unmodified Bio-Oss group and control group.No significant difference in cell DNA concentration between modified and unmodified Bio-Oss after 1d,3d,5dand 7dculture,but lower than control group.On the 14 dosteogenesis inducing,the ALP activity of the cultured MC3T3-E1 cells on modified Bio-Oss was higher than unmodified Bio-Oss group and control group.Conclusion：RGD peptide could enhance MC3T3-E1 cells adhesion on Bio-Oss and promote osteogenesis differentiation,but no obvious proliferation effects.

关 键 词：RGD多肽 无机小牛骨 MC3T3-E1 细胞黏附 

分 类 号：R782[医药卫生—口腔医学]