表达谱基因芯片筛选结直肠癌转移中Tiam1相关基因  

Screening of Tiam1-related genes contributing to metastasis of colorectal cancer using a gene expression profiling chip

作　　者：肖雨[1] 于莉娜[1] 丁彦青[1] 

机构地区：[1]南方医科大学基础医学院病理系/广东省分子肿瘤病理重点实验室,广州510515

出　　处：《临床与实验病理学杂志》2015年第4期363-367,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(81472319)

摘　　要：目的筛选在结直肠癌远处转移中T细胞淋巴瘤侵袭转移1(T-cell lymphoma invasion and metastasis 1,Tiam1)相关基因并鉴定其表达。方法腹腔注射二甲基肼(dimethylhydrazine,DMH)建立小鼠结直肠癌模型,收集Tiam1转基因小鼠和野生型小鼠的结直肠癌原发灶新鲜组织5对,应用Affymetrix小鼠全基因组表达谱芯片检测两组的差异表达基因,qRT-PCR技术验证部分差异基因以确定结果的可靠性。结果基因芯片检测结果显示差异在3倍以上的基因共794个,有远处转移的Tiam1转基因小鼠结直肠癌组织中表达上调的基因共400个,下调基因共394个;通过生物信息学分析及GENEMANIA共表达网络构建,筛选3个(PIK3R5、FIGF、RPS6KA6)特异相关的差异基因进行qRT-PCR技术验证,验证结果与基因芯片结果检测相符。结论通过基因芯片技术筛选和鉴定结直肠癌转移中Tiam1相关基因,建立了特异性结直肠癌转移表达谱,为寻找结直肠癌远处转移的分子标志物提供依据。Purpose To screen and identify T cell lymphoma invasion and metastasis 1 （Tiaml） related genes and expression profile during distant metastasis of eolorectal cancer. Methods Colorectal cancer（CRC） in a mice model was established by intraperitoneal injection of dimethylhydrazine （DMH）. Five panels of fresh primary tumor tissues from Tiaml transgenic mice were collected and com- pared with that from wild type mice. Differentially expressed genes were detected by Affymetrix human genome-wide expression profile chip and verified by real-time quantitative PCR （qRT-PCR） analysis. Results The geneehip result showed that 794 genes were differ- entially expressed by at least 3 folds in Tiaml transgenie mice with distant metastasis of coloreetal cancer tissue, including 400 up-regu- lated and 394 down-regulated ones. Bioinformatics analysis and gene co-expression network building identified 3 genes （ PIK3R5, FIGF and RPS6KA6） with specific expression in colorectal cancer tissue with distant metastasis, and this result was confirmed by qRT-PCR. Conehmion A specific Tiaml gene expression profile related to eolorectal cancer distant metastasis has been established through screening and identifying the genes related to Tiaml by gene chip technique. These findings provide a basis for exploring the molecular markers of coloreetal cancer with distant metastasis.

关 键 词：结直肠肿瘤 癌转移 TIAM1 基因芯片 PI3K/AKT/mTOR信号通路 

分 类 号：R735.3[医药卫生—肿瘤]