T细胞性淋巴瘤诊断中TCR基因重排检测的应用  

Analysis of TCR gene rearrangements for diagnosis of T-cell lymphomas

作　　者：潘鑫艳[1] 杨长绍[1] 黎贵芸[1] 杨举伦[1] 王丽[1] 

机构地区：[1]成都军区昆明总医院病理科,昆明650032

出　　处：《临床与实验病理学杂志》2015年第4期400-403,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(81201759);云南省应用基础研究(2012FB210)

摘　　要：目的探讨T细胞受体(T cell receptors,TCR)基因重排检测对T细胞性淋巴瘤诊断的价值。方法收集T细胞性淋巴瘤30例和淋巴反应性增生组织30例,提取DNA,应用BIOMED-2引物系统中的56条引物进行PCR扩增,核酸分子异源双链凝胶电泳分析结果。结果 30例T细胞性淋巴瘤标本中TCRβ、TCRγ、TCRδ的检出率分别为83.3%(25/30)、93.3%(28/30)、13.3%(4/30),三者联合检测的检出率为96.7%(29/30),30例淋巴反应性增生组织中均未检测出TCR基因重排。结论利用BIOMED-2引物系统检测TCR基因重排可作为T细胞性淋巴瘤的辅助诊断工具。Purpose To discuss the TCR gene rearrangements in the diagnosis of T-ceU lymphomas. Methods Formalin-fixed and paraffin-embedded samples including 30 cases of T-cell lymphomas and 30 cases of reactive lymphoid hyperplasia were chosen for ex- tracting genomic DNA and PCR amplification using 56 BIOMED-2 primers. PCR products were analyzed by heteroduplex and polyacryl- amide gel electrephoresis. Results In all 30 cases of T-cell lymphomas, 25 cases （83.3%） showed TCRβ gene monoclonal rear- rangements, 28 cases （93.3%） of TCRγ gene monoclonal rearrangements, 4 cases （13.3%） of TCR8 gene mcnoclonal rearrange- ments. 29 cases （96. 7% ） with TCRβ＋TCRγ＋TCRδ gene monoclonal rearrangements were detected, but no clonal TCR gene rear- rangements were found in 30 cases of reactive lymphoid hyperplasia. Conclusions The detection of TCR gene rearrangements using BIOMED-2 primers is a useful assistant method for the diagnosis of T-cell lymphomas.

关 键 词：T细胞性淋巴瘤 基因重排 BIOMED-2 TCR基因 

分 类 号：R733.4[医药卫生—肿瘤]