miR-584对人胶质母细胞瘤侵袭和迁移能力的影响  

Effects of miR-584 on the invasion and migration of human glioblastoma U251 cells

作　　者：韩晓[1] 薛皓[1] 闫绍峰[1] 郭兴[1] 李彤[1] 郭小凡[1] 高校 李刚[1] 

机构地区：[1]山东大学齐鲁医院神经外科,山东济南250012

出　　处：《山东大学学报（医学版）》2015年第4期16-21,共6页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81372719);国家自然科学基金青年基金(81101594)

摘　　要：目的探讨miR-584对人胶质母细胞瘤U251细胞侵袭和迁移能力的影响。方法将化学合成的miR-584-3p在脂质体Lipofectamine 2000的介导下,瞬时转染人胶质母细胞瘤U251,细胞分为空白对照组(无转染,其他条件相同)、mimics NC组(转染miR-584-3p mimics阴性对照序列)、miR-584-3p mimics组(转染miR-584-3p mimics)、inhibitor NC组(转染miR-584-3p inhibitor阴性对照序列)和miR-584-3p inhibitor组(转染miR-584-3p inhibitor)。Real-time PCR法检测细胞中miR-584-3p的表达水平;CCK-8法检测细胞增殖能力;划痕实验和Transw ell小室实验检测细胞的迁移和侵袭能力。结果与空白对照组和mimics NC组相比,miR-584-3p mimics组的miR-584-3p表达上调约100倍(P<0.05),细胞增殖能力无明显差异(P>0.05),细胞侵袭和迁移能力明显下降(P<0.05);与空白对照组和inhibitor NC组相比,miR-584-3p inhibitor组的miR-584-3p表达下调至空白对照组的5%(P<0.05),细胞增殖能力无明显差异(P>0.05),细胞侵袭和迁移能力显著增强(P<0.05)。结论 miR-584-3p mimics转染抑制了U251细胞的侵袭和迁移能力;miR-584-3p inhibitor转染能增强U251细胞的侵袭和迁移能力。Objective To explore the effect of miR-584 expression on the invasion and migration of glioblastoma U251 cells in vitro.Methods Chemosynthetic miR-584-3p was transfected into glioblastoma cells under the induction of Lipofectamine 2000.The cells were divided into control group（no transfection）,mimics NC group（transfected with negative control sequence of miR-584-3p mimics）,miR-584-3p mimics group（transfected with miR-584-3p mimics）, inhibitor NC group（transfected with negative control sequence of miR-584-3p inhibitor）,miR-584-3p inhibitor group （transfected with miR-584-3p inhibitor）.The expression of miR-584-3p was detected by Real-time PCR.Cell prolifera-tion was detected by CCK-8 proliferation assay.The invasive and migratory effects of glioblastoma cell were measured by Wound healing and Transwell assays.Results Compared with mimics NC group and control group,the expression of miR-584-3p in miR-584-3p mimics group was upregulated by 100 times（P 〈0.05 ）.Compared with mimics NC group and control group,the cell proliferation ability of miR-584-3p mimics group showed no significant change（P 〉0.05）,but the cellular migratory and invasive activity of miR-584-3p mimics group were down-regulated significantly （P 〈0.05）.Compared with inhibitor NC group and control group,the expression of miR-584-3p in miR-584-3p inhibi-tor group was down-regulated by 20 times（P 〈0.05）.Compared with inhibitor NC group and control group,the cell proliferation ability of miR-584-3p inhibitor group showed no significant change（P 〉0.05）,the cellular migration and invasive activity in miR-584-3p inhibitor group were up-regulated significantly（P 〈0.05）.Conclusion The migration and invasion of glioblastoma cells can be inhibited by the over-expression of miR-584-3p,while the transfection of miR-584-3p inhibitor can enhance the migration and invasion of glioblastoma cells.

关 键 词：miR-584 胶质母细胞瘤 侵袭 迁移 MiR-584 

分 类 号：R739.41[医药卫生—肿瘤]