乳腺癌TUSC1基因启动子的甲基化及其蛋白表达的相关性  

Promoter Methylation and Expression of TUSC1 Gene in Breast Cancer

作　　者：夏琬君[1] 程敬亮[1] 葛新[2] 路彦涛[3] 

机构地区：[1]郑州大学第一附属医院磁共振科,河南郑州450052 [2]郑州大学第一附属医院乳腺外科,河南郑州450052 [3]郑州大学第一附属医院肝胆胰外科,河南郑州450052

出　　处：《医学与哲学（B）》2015年第3期74-76,共3页Medicine & Philosophy(B)

摘　　要：为探讨乳腺癌中TUSC1基因启动子甲基化及其与蛋白表达的相关性。运用甲基化特异性聚合酶链反应及Western blot检测65例浸润性乳腺癌及其对应的癌旁组织中启动子甲基化及蛋白的表达。癌旁组织均未出现TUSC1基因启动子的甲基化,对应的乳腺癌组织中61.5%出现甲基化。癌旁组织TUSC1蛋白均表达,对应的乳腺癌组织29.2%出现蛋白表达。并且甲基化发生率与蛋白表达均与TNM分期及淋巴结转移有关(P<0.05)。TUSC1基因启动子甲基化是基因失活的重要机制之一,与乳腺癌的发生及发展有关。To study the relationship between status of methylation of TUSC 1 gene promoter and its protein in breast cancer . Methylation‐specific PCR and Western blot were applied respectively to examine the methylation of TUSC 1 gene promoter and its mRNA expression of 65 samples of invasive breast cancer and their matched adjacent non‐cancerous tissues .The results revealed that there was no methylation of TUSC1 gene promoter in adjacent non‐cancerous tissues ,while in the breast cancer tissues , the hyper‐methylation rate was 61 .5% . The result of Western blot revealed that the positive expression rate of TUSC1 protein was 100% in adjacent non‐cancerous tissues and 29 .2% in breast cancer tissues .In additional ,methylation of TUSC1 gene promoter and protein expression were related to the tumor TNM stage and lymph node metastasis （P〈 0 .05） .Methylation of promoter might be one of the important mechanisms of inactivation of TUSC 1 gene ,and might play an important role in carcinogenesis and progression of breast cancer .

关 键 词：乳腺癌 甲基化 肿瘤抑制因子候选基因1 

分 类 号：R737.9[医药卫生—肿瘤]