肉桂鞣质B-1对脊髓星形胶质细胞增殖的调节作用及对血清氧葡萄糖缺乏/复氧诱导细胞凋亡的保护作用  

Cinnamtannin B-B-1 regulates cell proliferation of spinal cord astrocytes and protects the cells from oxygen-glucose-serum deprivation/reoxygenation-induced apoptosis

作　　者：崔国峰[1] 武军龙[2] 毕郑刚[1] 李福春[1] 

机构地区：[1]哈尔滨医科大学附属第一医院骨科,哈尔滨150001 [2]郑州大学附属洛阳市中心医院骨科,河南洛阳471009

出　　处：《中国骨与关节外科》2015年第1期71-77,共7页Chinese Journal of Bone and Joint Surgery

基　　金：黑龙江省卫生计生委科研课题(2014-272);2015哈尔滨医科大学附属第一医院科研基金

摘　　要：背景背景:中枢神经系统中星形胶质细胞对神经元起重要的保护作用。据报道,一些抗氧化剂可以保护缺血/再灌注引起的星形胶质细胞功能障碍。肉桂鞣质B-1(Cinnamtannin B-1)是一种天然存在的A型原花青素,具有抗氧化作用。目的:研究肉桂鞣质B-1对脊髓星形胶质细胞的作用。方法:星形胶质细胞血清氧葡萄糖缺乏(OGSD)8 h后用或不用肉桂鞣质B-1进行复氧(R)。结果结果:肉桂鞣质B-1对OGSD/R诱导的星形胶质细胞凋亡具有保护作用,同时具有促进星形胶质细胞增殖的作用,而细胞外调节蛋白激酶(ERK)抑制剂逆转这种作用。结论结论:肉桂鞣质B-1通过ERK信号通路促进星形胶质细胞增殖,保护OGSD/R诱导的细胞凋亡。在中枢神经系统,肉桂鞣质B-1作为一种抗氧化剂对星形胶质细胞缺血/再灌注损伤具有保护作用。Background： Astrocytes are important for protecting neurons in central nervous system. It has been reported that some antioxidants could protect astrocytes from ischemia- reperfusion- induced dysfunction. Cinnamtannin B- 1 is a naturally occurring type-A proanthocyanidin that exhibits antioxidant properties.Objective： The purpose of the study is to investigate the effects of cinnamtannin B-1 on spinal cord astrocytes.Methods： Astrocytes were subjected to oxygen- glucose- serum deprivation for eight hours followed by reoxygenation with or without cinnamtannin B-1.Results： Cinnamtannin B-1 protected astrocytes from oxygen-glucose-serum deprivation and reoxygenation-induced apoptosis. Concurrently, cinnamtannin B-1 promoted the proliferation of astrocytes whereas the extracellular regulated protein kinase（ERK） inhibitor reversed this effect.Conclusions： Cinnamtannin B-1 protects astrocytes from oxygen-glucose-serum deprivation/reoxygenation-induced apoptosis by promoting astrocyte proliferation via an ERK pathway. Therefore, as an antioxidant, cinnamtannin B-1 may provide extra benefit for astrocyte protection during ischemia-reperfusion in the central nervous system.

关 键 词：星形胶质细胞 肉桂鞣质B-1 缺血/再灌注 细胞凋亡 

分 类 号：R744[医药卫生—神经病学与精神病学]