一株抗菌活性物质产生细菌的分离及鉴定  

Isolation and Identification of One Strain Producing Antimicrobial Active Substance

作　　者：殷杰[1] 王蒙[1] 曾小波[1] 马志勇[1] 程国军[1] 

机构地区：[1]中南民族大学生命科学学院,武汉430074

出　　处：《湖北农业科学》2015年第2期346-349,共4页Hubei Agricultural Sciences

基　　金："十二五"国家级中南民族大学民族药学实验教学中心建设项目;国家级大学生创新创业训练计划项目(GCX12011)

摘　　要：从根际土壤分离到一株细菌CY04,通过电镜观察和16S r DNA序列分析,发现该菌株同已知菌枯草芽孢杆菌(Bacillus subtilis)的相似度最高,为99%,初步认定为枯草芽孢杆菌。抑菌试验结果表明,该菌株对金黄色葡萄球菌(Staphylococcus aureus)有一定的抑菌活性,对大肠杆菌(Escherichia coli)和枯草芽孢杆菌没有抑菌活性。培养48 h左右是其产生抗菌活性物质的最佳时期。A bacterium strain, designated as CY04, was isolated from the rhizosphere soil. The strain CY04 had the highest sequence identity with Bacillus subtilis（99%）. Based on morphological characteristics and analysis of the 16 S r DNA sequence, the strain CY04 was identified as Bacillus subtilis. The antagonistic effects showed that CY04 had antimicrobial activity on Staphylococcus aureus, but had little inhibition on Escherichia coli and Bacillus subtilis. The optimal time for the resistant bacteria to produce antimicrobial active substance was 48 h after cultivation.

关 键 词：根际土壤 枯草芽孢杆菌(Bacillus subtilis) 鉴定 抑菌活性 

分 类 号：Q936[生物学—微生物学]