扶正散结汤拆方配伍中药抑制Lewis肺癌生长和对肿瘤细胞周期的影响  

Effect of Fuzheng Sanjie Decoction and its compositions on tumor growth and cell cycle of transplanted Lewis lung carcinoma

作　　者：窦永起[1] 陈慧彬[1] 陈超[1] 

机构地区：[1]中国人民解放军总医院中医科,北京100853

出　　处：《环球中医药》2015年第3期320-323,共4页Global Traditional Chinese Medicine

摘　　要：目的研究扶正散结汤拆方抑制Lewis肺癌荷瘤小鼠移植瘤生长和对组织形态与细胞周期影响的差异与协同作用。方法 C57BL/6小鼠80只,随机分为:空白组、模型组、顺氯氨铂组、益气扶正组、化痰散结组、活血化瘀组、清热解毒组、中药联合组,每组10只。除空白组外,其余小鼠建立肺癌移植瘤模型。接种后第2天开始用药,连续用药14天,于接种后第15天处死全部小鼠,测量小鼠的瘤重、组织HE染色观察和采用流式细胞仪分析肿瘤细胞周期及凋亡情况。结果与模型组比较,其余各组瘤重均显著降低(P<0.05),各中药组对Lewis肺癌的抑制作用以联用组最为明显,抑瘤率最高;各组肿瘤细胞在G0/G1期比例,与模型组比较各组均增高(P<0.05),凋亡率显著增加(P<0.01),化痰组及解毒组在G0/G1期比例高于联用组(P<0.05),但联用组凋亡率最高;各组肿瘤细胞在G2/M期的比例,除扶正组及活血组外,其余各中药组均明显减少(P<0.05);组织学观察示各组均较模型组细胞稀疏,呈散在、条索状分布,均可见不同程度的片状坏死区及核固缩、核破碎溶解的现象。结论化痰组及解毒组单独应用时阻滞肿瘤细胞分裂的效果更突出,但各组中药联合应用对抑制肿瘤生长效果更明显,其机制与阻滞肿瘤细胞分裂,同时还可促进肿瘤细胞坏死及诱导肿瘤细胞凋亡有关。Objective To study the inhibition of Fuzheng Sanjie Decoction and its compositions on the growth of tumors , and the effect on tissue morphology and cell cycle. Method Totally 80 C57BL/ 6 mice were randomly assigned into 8 groups including the blank group, model group, DDP group, Yiqi Huoxue group, Huatan Sanjie group, Huoxue Huayu group, Qingre Jiedu group and combined-therapy group with 10 mice in each group. The model of Lewis transplanted tumor was established except the blank group. Each group was intragastric administration one time every day for 14 days after the transplantation. Then the mice were sacrificed to measure the weight of tumor and observe tumor pathology, cell cycle and apoptosis. Result Compared with the model group, the weight of tumors in other groups were decreased significantly （ P 〈 0.05 ）. The tumor inhibition rate and apoptosis rate of the combined-therapy group were the highest. Compared with the model group, G0/G1 phase and apoptosis rate were increased in other groups （P 〈 0. 05 and P 〈 0.01, respectively）. The G0/G1 phase ratios of Huatan group and Jiedu group in were both higher than the combined-therapy group. The proportion of tumor cells in G2/M phase of all the groups except Fuzheng and Huoxue was significantly reduced（P 〈 0. 05 ）. According to the histological observation, cells of medicated groups were more sparsely distributed. Morphological changes including necrosis area, karyopyknosis, nuclear fragmentation and nucleus dissolved in the tumors could be observed under inverted microscope. Conclusion The effect on blocking tumor cell division were more outstanding in Huatan group and Jiedu group. But the inhibition on tumor growth was more obvious in the combined-therapy group, of which the mechanism was associated with blocking tumor cell division, promoting tumor cell necrosis and inducing tumor cell apoptosis.

关 键 词：扶正散结方 Lewis移植瘤 细胞周期 细胞凋亡 

分 类 号：R734.2[医药卫生—肿瘤]