白内障患者外周血中一氧化氮合酶的表达及其临床意义  

Change of nitric oxide synthase in peripheral blood of cataract patients

作　　者：叶荷花[1] 张济明[2] 陆云峰[2] 钱益勇[2] 

机构地区：[1]上海交通大学医学院附属新华医院眼科,200092 [2]苏州大学附属第一医院眼科,215006

出　　处：《中华实验眼科杂志》2015年第4期351-355,共5页Chinese Journal Of Experimental Ophthalmology

基　　金：苏州大学青年教师自然科学基金项目（Q312203010）

摘　　要：背景 已有研究表明,白内障患者晶状体和房水中一氧化氮合酶（NOS）含量增加,但检测较为困难,而确定人体血液中NOS的过度表达是否与白内障有关对于白内障的临床检测和早期预防具有重要意义.目的 探讨白内障患者外周血中NOS的表达变化及其临床意义.方法 采用前瞻性队列研究设计,选择2014年1-6月在苏州大学附属第一医院及上海交通大学医学院附属新华医院眼科就诊的年龄相关性白内障患者25例、糖尿病性白内障患者12例和同期体检的健康者20名,3个组患者基线特征匹配.收集所有受检者的外周血样本2.0 ml,离心法获得沉淀细胞.分别采用逆转录PCR（RT-PCR）法和流式细胞术检测受检者外周血中总NOS mRNA和诱导型NOS（iNOS） mRNA及其蛋白的表达水平,对各组间的检测结果进行比较.结果 年龄相关性白内障组、糖尿病性白内障组和健康对照组受检者外周血细胞中总NOS mRNA的相对表达值分别为1.46±0.31、1.59±0.28和1.22±0.23,3个组间的总体比较差异有统计学意义（F=7.572,P=0.001）,其中年龄相关性白内障组、糖尿病性白内障组受检者外周血中NOS mRNA的相对表达值明显高于健康对照组,差异均有统计学意义（q=4.095、5.170,均P＜0.01）;年龄相关性白内障组、糖尿病性白内障组和健康对照组受检者外周血细胞中iNOS mRNA的相对表达值分别为1.17±0.21、1.08±0.15和0.89±0.17,3个组间的总体比较差异有统计学意义（F=12.801,P=0.000）,其中年龄相关性白内障组、糖尿病性白内障组受检者外周血中iNOS mRNA的相对表达值明显高于健康对照组,差异均有统计学意义（q=7.112、3.932,均P＜0.01）.年龄相关性白内障组、糖尿病性白内障组和健康对照组受检者外周血细胞中总NOS蛋白的相对表达值分别为15.82±2.11、17.19±3.37和10.63±2.28,总体比较差异有统计学意义（F=34.895,P=0.000）,其中年龄相关性白内障组Background Studies showed that the content of nitric oxide synthase （NOS） in aqueous and lenses elevates in cataract eyes.But it is difficult to measure the NOS content in eye tissue.So to determine whether the overepression of NOS is associated with cataractogenesis is significant for the prevention and treatment of cataract.Objective This study was to detect the expression of NOS in the peripheral blood of cataract patients and evaluate the association of blood NOS with cataractogenesis.Methods Twenty-five patients with age-related cataract and 12 patients with diabetic cataract were collected from January to June 2014 in Affiliated First Hospital of Soochow University and Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine,and 20 matched healthy volunteers were enrolled in the Health Check Centre in the same duration.The 2.0 ml peripheral blood was collected from each subject under the informed consent.The blood specimens were centrifuged to obtain cell pellets.The contents of NOS mRNA and inducible nitric oxide synthase （iNOS） mRNA and their proteins in leukocytes were measured by reverse transcription PCR （RT-PCR） and flow cytometry.Results The relative expression levels of total NOS mRNA were 1.46±0.31,1.59±0.28 and 1.22±0.23 in the age-related cataract group,the diabetic cataract group and the healthy control group,respectively,with a significant difference among the three groups （F =7.572,P =0.001）.The relative expression level of total NOS mRNA was significantly higher in the age-related cataract group and the diabetic cataract group than that of the healthy control group （q =4.095,5.170,both at P＜0.01）.The relative expression levels of iNOS in blood were 1.17±0.21,1.08±0.15 and 0.89±0.17 in the age-related cataract group,the diabetic cataract group and the healthy control group,respectively,showing a significant difference among the three groups （F =12.801,P =0.000）,and the level of iNOS in blood was significantly increased in the agerelated cata

关 键 词：一氧化氮合酶 一氧化氮 白内障 外周血 逆转录PCR 流式细胞术 

分 类 号：R776.1[医药卫生—眼科]