荧光蛋白芯片方法检测肝癌血清中的AFP  

Detecting AFP serum level by using Fluorescence protein microarray

作　　者：尹成增 张爱英[2] 赵元顺[1] 林栋栋[1] 李宁[1,2] 

机构地区：[1]首都医科大学附属北京佑安医院,北京100069 [2]首都医科大学附属北京佑安医院,北京市肝病研究所,北京100069

出　　处：《中国医学装备》2014年第B12期126-127,共2页China Medical Equipment

基　　金：首都医科大学重点实验室基金(2013GYGA03);国家国际科技合作专项<中美英联合乙肝相关肝癌早期诊断分子标志物筛查及免疫机制研究>(项目批号S2012ZR0153);北京市卫生系统高层次卫生技术人才培养计划(项目批号2013-3-074);重大传染病防治协同创新中心资助

摘　　要：目的:研发一种简单、有效、低成本荧光蛋白芯片方法,检测血清中的甲胎蛋白(AFP),用于原发性肝细胞癌(HCC)的诊断。方法:建立AFP抗体蛋白芯片,检测46份肝癌血清、32份正常健康人血清,荧光扫描对检测结果进行判定。结果:31份正常人血清AFP含量低于20ng/ml,1份正常人血清的AFP含量为20ng/ml;46份肝癌血清的AFP水平均高于20ng/ml。结论:成功的建立了血清AFP的荧光蛋白芯片检测方法。Ojective： This study was aimed to develop a simple, effective, low-cost Fluorescence Protein microarray to detect serum AFP level for hepatocellular carcinoma （HCC） diagnosis. Methods： A antibody mieroarray for AFP determination has been developed. Then, 46 suspected serum samples of hepatocellular carcinoma and 32 healthy serum samples were tested by this method. Results： Thirty one of 32 Healthy controls had serum AFP levels under 20 nanograms per milliliter of blood （ng/ml） and only one healthy control had serum AFP levels was 20ng/ml. All the 46 patients had serum AFP levels above 20ng/ml. Conclusion： A Fluorescence protein microarray method was established for detection of AFP in serum.

关 键 词：荧光蛋白芯片 甲胎蛋白 肝癌 

分 类 号：R735[医药卫生—肿瘤]