不同动脉阻塞及阻塞时限对大鼠局灶性脑缺血模型的病理及行为学影响  

The Effect of Different Types of Arteries Occluded and Occlusion Time on Pathologyand Behavior Outcome in Focal Cerebral Ischemia Rat

作　　者：王骏阳[1,2] 李九如[1,2] 周余蕾 金坤林 邵蓓[1,2] 

机构地区：[1]温州医科大学附属第一医院神经内科 [2]浙江省高校重点学科神经生物学,325000

出　　处：《中国临床神经科学》2015年第2期130-137,共8页Chinese Journal of Clinical Neurosciences

基　　金：浙江省教育厅科研项目(编号:Z201016125)

摘　　要：目的通过采用不同的动脉阻塞和阻塞时限建立大脑中动脉(MCA)远端阻塞(d MCAO)的脑缺血模型,比较脑梗死体积及行为学评分的差异,并确定最佳的阻塞动脉方式和阻塞时限。方法第1阶段:在相同阻塞时限(90 min)下,采用不同动脉阻塞方法建立脑缺血模型:1电凝一侧大脑MCA远端(MCAO组,n=10);2电凝MCA+同侧颈总动脉(CCA)阻塞90 min(MCAO+1CCAO*90组,n=10);3电凝MCA+双侧CCA阻塞90 min(MCAO+2CCAO*90组,n=10);另设对照组(MCA和CCA皆不阻塞,n=8)。造模24 h后检测脑梗死体积和行为学评分。第2阶段:采用电凝MCA+双侧CCA阻塞法,阻塞时限分别为30 min(MCAO+2CCAO*30组,n=10)、60 min(MCAO+2CCAO*60组,n=10)、120 min(MCAO+2CCAO*120组,n=10)建立脑缺血模型,于造模24 h后检测梗死体积和行为学评分。结果在相同阻塞时限(90 min)下,MCAO+2CCAO*90组的脑梗死体积和运动功能缺损较MCAO组及MCAO+1CCAO*90组明显,且差异有显著统计学意义(P<0.01)。MCAO组与MCAO+1CCAO*90组比较差异无统计学意义(P>0.05)。在阻塞相同动脉(电凝MCA+双侧CCA阻塞)条件下,MCAO+2CCAO*120组死亡率高达60%;MCAO+2CCAO*60组的脑梗死体积和运动功能缺损与MCAO+2CCAO*30组比较,差异有显著统计学意义(P<0.01)。MCAO+2CCAO*60组与MCAO+2CCAO*90组比较,差异无统计学意义(P>0.05)。结论电凝一侧MCA+60 min一过性阻塞双侧CCA制作d MCAO模型可产生相对明显的梗死体积和运动功能损伤,且死亡率低、造模时间短,适用于缺血性脑卒中研究。Aim To compare the differences of infarct volume and neurological behavior outcome in distal middle cerebral artery occlusion （dMCAO） model established with different arteries occluded and occlusion time, and then optimize the arteries occluded and occlusion time. Methods Three focal cerebral infarctions were induced for 90 min due to the three kinds of occlusion：the distal middle cerebral artery （MCA） alone （MCAO group, n=10）; MCA plus ipsilateral common carotid artery （CCA）（MCAO＋1CCAO＊90 group, n=10 ）;and MCA plus bilateral CCA （MCAO＋2CCAO＊90 group, n=10）. Sham-operated group （n=8） was also included. Infarction volume was measured and behavior tests were performed in 24 h after cerebral ischemic. Another three focal cerebral infarctions were induced due to the occlusion of MCA plus bilateral CCA for 30 min （MCAO＋2CCAO＊30 group, n=10）, 60 min （MCAO＋2CCAO＊60 group, n=10）;120 min （MCAO＋2CCAO＊120 group, n=10） respectively. Then the infarction volume and behavior tests were compared along with MCAO＋2CCAO＊90 group. Results Under the circumstance of same occlusion time （90 min）, the infarction volume and motor deficit in MCAO＋2CCAO＊90 group was more obvious than MCAo and MCAO＋1CCAO＊90 group and there was signiifcant difference between them （P〈0.01）. There were no significant differences between MCAO and MCAO＋1CCAO＊90 group （P〉0.05）. Under the condition of same arteries occluded （MCAO＋2CCAO） but in different occlusion time, our study demonstrated that MCAo＋2CCAO＊120 produced a 60%morality rate. The infarction volume and motor deficit in MCAO＋2CCAO＊60 group was more obvious than MCAO＋2CCAO＊30 group and there was significant difference between them （P〈0.01）. However, there were no significant differences between MCAO＋2CCAO＊60 and MCAo＋2CCAO＊90 group. Conclusion Our results suggested that bilateral CCA occlusion for 60 min accompanied by permanent distal middle cerebral artery occlusion is a conven

关 键 词：局灶性脑缺血模型 大脑中动脉 行为学 梗死体积 阻塞时限 

分 类 号：R743[医药卫生—神经病学与精神病学] Q189[医药卫生—临床医学]