侧脑室注射4,4＇-二异硫氰基芪-2，2＇-二磺酸对大鼠脑缺血再灌注损伤的影响  

Protective Effects and Mechanisms of 4,4＇-diisothiocyanostibibene-2,2＇- disulfonic Acid Injected by Intarcerebroventricutar against Cerebral Ischemia-reperfusion Injury Rat

作　　者：袁征[1] 张璐[2] 武文卿[1] 袁子彦[2] 赵善民[2] 余琛琳[2] 林丽芳[2] 汤球[2] 

机构地区：[1]军事医学科学院科技部条件处,北京100036 [2]第二军医大学实验动物中心,上海200433

出　　处：《实验动物与比较医学》2015年第2期92-96,共5页Laboratory Animal and Comparative Medicine

基　　金：上海市科委发展基金（121409009700）

摘　　要：目的探讨侧脑室注射4，4’-二异硫氰基芪-2，2’-二磺酸（DIDS）对脑缺血再灌注（CIRI）大鼠脑组织损伤的保护作用及其机制。方法采用线栓法制备大鼠大脑中动脉栓塞（MCAO）模型，侧脑室注射法给药。脑缺血2h再灌注24h后用Zea—Longa标准进行神经功能评分；红四氮唑溶液（TTC、染色法测定大鼠脑梗死面积；Westernblot法测大鼠脑组织B淋巴细胞瘤．2（B—celllymphoma-2，Bcl-2）和天冬氨酸蛋白酶．3（Caspase-3）两种蛋白的表达水平；试剂盒测定大鼠脑组织内诱导型一氧化氮合酶（iNOS）和谷胱甘肽过氧化物酶（GSH—PX）的活性。结果100gmol／LDIDS侧脑室给药能改善大脑的神经功能，减少脑缺血面积，增加Bcl-2的表达，减少Caspase-3的表达，降低iNOS的活性。结论DIDS可以通过调节凋亡相关蛋白的表达以及降低iNOS的活性对大鼠神经细胞进行保护，减轻脑缺血再灌注损伤（CIRI）造成的脑组织损伤。Objective To explore the protective effects and mechanisms of 4,4＇-diisothiocyanostibibene- 2,2＇-disulfonic acid （DIDS） injected by intarcerebroventricular against cerebral ischemia-reperfusion injury （CIRI） in middle cerebral artery occlusive （MCAO） rats. Methods Focal cerebral ischemia in rats was produced by 2 h occlusion of the middle cerebral artery and 24 h reperfusion. DIDS and normal saline （NS） was injected by intarcerebroventricular. The neurologic deficit scores were investigated according to Zea-Longa＇s Standard. The infarct areas were assessed with software Imagepro Plus 6.0 after TTC staining. The levels of Bcl-2 and Caspase-3 in central neutral system were assessed by west- ern blot. And the activities of inducible nitric oxide synthase （iNOS） as well as glutathione peroxidase （GSH-PX） were determined by specific kit. Results DIDS （100 p.mol/L） could decrease the neurologic deficit score, reduced the infarct areas of the brain in CIRI rats, enhance the expression of Bcl-2, depresse the expression of Caspase-3, and decrease the activity of iNOS. Conclusion DIDS has a protective effect on CIRI of rat and this effect is related to enhancing the expression of Bcl-2 and diminishing Caspase-3 expression, decreasing the activity of iNOS.

关 键 词：缺血再灌注损伤(CIRI) 侧脑室注射 4 4’-二异硫氰基芪-2 2’-二磺酸(DIDs) B淋巴细胞瘤(Bcl-2) 天冬氨酸蛋白酶.3(Caspase-3) 诱导型一氧化氮合酶(iNOS) 谷胱甘肽过氧化物酶(GSH—PX) 

分 类 号：R743.3[医药卫生—神经病学与精神病学]