长春七根部挥发油的测定及抑菌活性研究  

Analysis of Essential Oil from the Roots of Libanotis buchtormensis and Antibacterial Activity Test

作　　者：葛晓晓[1] 姚成[1] 边敏[1] 曹莉[2] 

机构地区：[1]南京工业大学理学院,江苏南京211816 [2]南京工业大学食品与轻工学院,江苏南京211816

出　　处：《南京师范大学学报（工程技术版）》2015年第1期67-72,共6页Journal of Nanjing Normal University(Engineering and Technology Edition)

摘　　要：研究了长春七根部挥发油的提取方法,结合该部位的抑菌活性测试,提高挥发油的综合利用价值.采用挥发器测定和索式抽提两种方法和不同溶剂分别对长春七根部进行挥发油的提取,通过GC-MS联用技术测定分析有效成分,并进行比对,同时测试了其对大肠杆菌和金黄色葡萄球菌的抑菌活性.结果表明,两种方法的提取率和提取成分的种类与含量存在差异.抑菌活性测试表明长春七根部挥发油对大肠杆菌和金黄色葡萄球菌均能产生明显的抑制作用.两种方法可以互补地用于长春七根部中的有效成分的提取,结合挥发油的抑菌活性,进一步明确了该部位的应用前景.The aim of this article is to improve comprehensive value of essential oils through studying the extraction methods of essential oils from roots of Libanotis buchtormensis（ Fisch） DC and the antibacterial activity test. The essential oils are extracted by steam distillation and soxhlet extraction with different solvents. The chemical constituents of essential oils are identified and analyzed by GC-MS. The colon bacillus and staphylococcus aurous are used for antibacterial activity test. There are differences in extraction rate,kinds and contents with the different methods and different solvents. The antibacterial activity test proves that propagate frequency of colon bacillus and staphylococcus aurous are obviously restrained by essential oils from roots of Libanotis buchtormensis（ Fisch） DC. Many compounds are isolated and identified for the first time. Effective constituents can be extracted by two methods in the general. Combining with the antibacterial activity test,essential oils from roots of Libanotis buchtormensis（ Fisch） DC will get a very wide application foreground.

关 键 词：长春七 挥发油 气相色谱-质谱分析 抑菌活性 

分 类 号：R284.1[医药卫生—中药学]