一株高环多环芳烃降解嗜盐菌Thalassospira sp.的分离及降解特性  

Isolation and degradation characteristics of a HMW-PAHs degrading halophilic strain Thalassospira sp.

作　　者：王慧[1] 周海燕[1] 黄勇[1] 方婷婷[1] 

机构地区：[1]清华大学环境学院,北京100084

出　　处：《清华大学学报（自然科学版）》2015年第1期87-92,共6页Journal of Tsinghua University(Science and Technology)

基　　金：水体污染控制与治理国家重大专项资助项目(2012ZX07301-001)

摘　　要：为获得盐环境中高环多环芳烃（HMW-PAHs）污染修复的高效降解菌株,该文以芘为唯一碳源和能源,利用5%盐度矿物培养基（MSM）从石油污染土壤中富集分离出一株能降解菲、荧蒽、芘、苯并蒽的嗜盐菌,经形态学观察和16SrRNA序列鉴定为海旋菌（Thalassospira）,命名为Thalassospira sp.strain TSL5-2。在5%盐度下,25d内,TSL5-2对菲、芘、荧蒽（初始质量浓度均为20mg/L）、苯并蒽（初始质量浓度为8 mg/L）的降解率分别为100%、53.3%、60%、18.1%,但不能降解苯并芘。TSL5-2耐盐范围为0.5%~19.5%,最适盐度为5%。外加酵母粉和蛋白胨（5mg/L）对菲的降解都有一定的促进作用,后者效果更为明显,5d内菲的降解率分别为60.9%和82.1%,高于MSM对照组（46.1%）。酵母粉对TSL5-2降解芘和苯并芘的影响效果都不显著。蛋白胨对芘降解有抑制作用,但对苯并芘（5mg/L）降解有促进效果,25d的芘和苯并芘的降解率分别为17.5%和38.2%。A halophilic cell strain was isolated that efficiently degrades high molecular weight-polycyclic aromatic hydrocarbon （HMW-PAHs） contamination in saline environments, This strain, which can degrade phenanthrene, fluoranthene, pyrene and benzanthraeene, was isolated from oil-polluted soil with pyrene as the sole carbon and energy source in a mineral salt medium （MSM） of 5% salinity. The strain was identified as Thalassospira based on morphological observations and an 16SrRNA sequence and named Thalassospira sp. strain TSLS-2. After 25 days＇ cultivation in 5 salinity culture solution, strain TSLS-2 degraded phenanthrene by 100%, pyrene by 53.5 %, fluoranthene by 60% （initial concentration： 20 rag/L） and benzanthracene by 18.1% （initial concentration： 8 mg/L）, but could not degrade benzopyrene. TSL5-2 had a wide salinity tolerance range （0.5% - 19.5%）, with an optimal salinity of 5%. Adding yeast powder and peptone （ 5 mg/L） to the MSM both improved the degradation of phenanthrene by TSLS-2, with degradation rates of 60.1% with yeast and 82.1% with peptone at day 5, both higher than the control group （46.1%）. Yeast powder did not effectively improve the degradation of pyrene and benzopyrene. Peptone inhibited the degradation of pyrene, while accelerating benzopyrene degradation, with a pyrene degradation rate of 17.5% and a benzopyrene （5 mg/L） degradation rate of 38.2% at 25 days.

关 键 词：芘 嗜盐菌分离 Thalassospira菌 降解 

分 类 号：X172[环境科学与工程—环境科学]