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作 者:田英杰[1] 孙进[1,2] 谢振兴[1] 马玉华[1] 乐国伟[1,2] 施用晖[1,2]
机构地区:[1]江南大学食品学院,江苏无锡214122 [2]食品科学与技术国家重点实验室,江南大学,江苏无锡214122
出 处:《食品与生物技术学报》2015年第3期267-273,共7页Journal of Food Science and Biotechnology
基 金:国家“十二五”科技支撑计划项目(2012BAD33B05);江苏高校优势学科建设工程资助项目
摘 要:探讨了高脂膳食小鼠补充γ-氨基丁酸(GABA)对骨性能的影响。40只6周龄C57BL/6雄性小鼠,随机分成4组:正常日粮组(control),高脂日粮组(HFD),高脂日粮小鼠饮水补充0.06、0.12g/dL GABA组。试验结束前一周收集各组小鼠尿液,测定钙及羟脯氨酸含量;第20周结束试验,测定小鼠股骨、胫骨参数,以及股骨氧化还原指标,荧光定量PCR检测相关基因表达。结果显示,与对照组相比,HFD组股骨出现氧化应激,股骨GAD65显著下调、GABAbR显著上调(P<0.05),GSK3β显著上调而Nrf2显著下调(P<0.05);股骨和胫骨钙含量、骨强度等相关性能指标显著降低,尿钙及羟脯氨酸含量显著增高,且成骨细胞分化标志基因BGLAP2、col1a1显著下调,破骨细胞分化标志基因RANKL/OPG、CTSK、TRAP显著上调(P<0.05)。补充0.06、0.12g/dLGABA可恢复GABAbR、GSK3β、Nrf2表达(P<0.05),减轻股骨氧化损伤,同时平衡骨细胞分化及代谢,提高股骨和胫骨性能。结果表明,GABA可调节股骨氧化还原状态,平衡骨代谢,改善股骨及胫骨性能;0.12g/dL GABA改善股骨氧化应激和股骨性能的作用优于0.06g/dL的。This manuscript was aimed to investigate the effect of γ-aminobutyric acid on bone performace in high-fat-diet-fed mice.For this,6-week-old C57BL/6 male mice(40) were randomly divided into four groups:normal diet group(C),high-fat diet group(HFD),mice in HFD supplemented with 0.06%,0.12% GABA by drinking water.A week prior to sacrifice,mice were placed in metabolism cages to collect urine for measuring urinary calcium and hydroxyproline content.Mice were sacrificed by 20^th,femur,tibia were collected for measuring parameters,femoral oxidative stress and relative genes expression by RT-PCR.The results demonstrated compared with the control group,HFD showed femoral oxidative stress,down-regulated GAD65 and increased GABAb R1 expression as well as dramatically significantly up-regulated GSK3β and down-regulated Nrf2;In addition,mice in HFD showed significantly reduced femoral and tibial performance,while dramatically increased urinary calcium and hydroxyproline content.At the same time,osteoblast-specific genes Col1a1,BGLAP2 significantly down-regulated while osteoclast-specific genes expression RANKL/OPG,TRAP,CTSK increased significantly in HFD.0.06%,0.12% GABA supplement can significantly recovered GSK3β,Nrf2,GABAb R1 expression,alleviated femoral oxidative stress,and thus improved femur and tibia performance accompanied by balanced bone metabolism.Conclusion:GABA can balance bone metabolism and partially recover bone performance by alleviating oxidative stress.0.12% GABA showed a better effect than 0.06% in adjusting femoral redox state and improving bone performance.
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