肝素/壳聚糖微胶囊的制备及其抗凝血性能  

Preparation of Heparin/Chitosan Microcapsule for Anticoagulation Study

作　　者：梁艳[1] 宋家鸿 陈荆晓[1] 陈敬华[1] 

机构地区：[1]江南大学药学院,江苏无锡214122

出　　处：《食品与生物技术学报》2015年第3期295-301,共7页Journal of Food Science and Biotechnology

基　　金：教育部博士点基金项目(20110093110008);江苏省自然科学基金项目(BK2012557)

摘　　要：采用层层自组装(LbL)技术制备了肝素/壳聚糖(HEP/CHI)5微胶囊,并研究了其抗凝血性能、胃肠道稳定性和毒副作用等。结果表明:该微胶囊具有球形结构;在p H 5.0、7.4中肝素能够缓慢可控地释放;在p H 5.0、7.4以及模拟胃液、肠液条件下具有稳定性;APTT法和全凝固实验验证了它在体外具有良好的抗凝血效果;并且对内皮细胞无细胞毒性。因此,该微胶囊有望作为口服剂型,用于临床肝素给药。Heparin/chitosan（HEP/CHI）5microcapsules for anticoagulation were prepared by Layerby-layer（LbL） technique.The stability of（HEP/CHI）5microcapsules under pH 5.0 and 7.4,simulated gastric and intestinal juices conditions was studied respectively,APTT anticoagulant time and cytotoxicity of microcapsules against endothelial cells were estimated.Results indicated that（HEP/CHI）5 microcapsules were of spherical structure and stable in different simulated physiological milieus;they could behave favorable anticoagulant efficacy with no significant cytotoxicity.Thus,（HEP/CHI）5 microcapsules were promising for clinical heparin administration as a novel oral dosage form.

关 键 词：微胶囊 肝素 抗凝血 层层自组装 口服剂型 

分 类 号：R943[医药卫生—药剂学] R96[医药卫生—药学]