陕西浓缩苹果汁中高渗酵母的分离鉴定  

Identification of Osmotolerant Yeast in Apple Juice Concentrate from Shaanxi Province

作　　者：王虎玄 岳田利[1] 胡仲秋[1] 牛晨[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《农业机械学报》2015年第4期246-251,共6页Transactions of the Chinese Society for Agricultural Machinery

基　　金：'十二五'国家科技支撑计划资助项目(2012BAD31B01)

摘　　要：为研究引起苹果浓缩汁污染腐败的高渗酵母,采用YPD高渗培养基对陕西苹果汁加工厂的苹果浓缩汁中的高渗酵母进行分离纯化,并对分离菌株的耐糖能力进行了研究。对分离菌株菌落形态、细胞形态、生理生化特征进行了分析,并以26SrDNA序列构建系统发育树,确定了7株分离菌的遗传学位置。进一步研究了分离株在苹果浓缩汁中的生长情况。除一株分离菌为热带假丝酵母(Candida tropicalis)外,其余分离株均为鲁氏接合酵母(Zygosaccharomyces rouxii)。鲁氏接合酵母(Z.rouxii)能够在葡萄糖质量浓度为900g/L的培养基中生长,耐糖能力明显强于热带假丝酵母(C.tropicalis),并且鲁氏接合酵母(Z.rouxii)也能够在苹果浓缩汁中生长。结果表明:鲁氏接合酵母(Z.rouxii)会对苹果浓缩汁质量造成威胁,需要加强控制。In order to study osmotolerant yeasts which are capable of causing spoilage for apple juice concentrate, the yeasts present in apple juice concentrate collected from apple juice processing plants in Shaanxi Province were isolated and purified by using osmotic YPD media. The sugar-tolerance of different yeast isolates was assayed. The colony morphology, cell morphology and physiological characteristics were studied and the phylogenetic tree based on 26S rDNA sequence analysis was constructed to determine the genetic location of isolates. Except the isolate N18 which was identified as strain of Candida tropicalis, all the other isolates belonged to Zygosaccharomyces rouxii. All the isolated strains of Z. rouxii were capable of growing in 900 g/L glucose and apple juice concentrate, displaying better sugar-tolerance ability and greater harm to the quality of apple juice concentrate than isolate of C. tropicalis which showed sugar-tolerance of 750 g/L glucose and no visible growth in apple juice concentrate. The results suggested that strains of Z. rouxii might pose a threat to the quality of apple juice concentrate, and it was needed to be controlled in the production line of apple juice concentrate.

关 键 词：苹果浓缩汁 高渗酵母 26S RDNA 鲁氏接合酵母 

分 类 号：S182[农业科学—农业基础科学]