MiRNA-122对5-氟尿嘧啶诱导BEL-7402/5-FU细胞凋亡的影响  

Effects of miRNA-122 on BEL-7402 /5-FU cell apoptosis induced by 5-fluracil

作　　者：虞佳[1] 唐正午[2] 杨晓燕[1] 殷杰[1] 向琼[1] 雷小勇[1] 

机构地区：[1]南华大学药物药理研究所,湖南衡阳421001 [2]南华大学附属第一医院骨科

出　　处：《中南医学科学杂志》2015年第2期137-140,共4页Medical Science Journal of Central South China

基　　金：湖南省教育厅一般课题(13C832)

摘　　要：目的探讨微水RNA-122(miR-122)对5-氟尿嘧啶(5-FU)诱导BEL-7402/5-FU细胞凋亡的影响。方法构建miR-122和阴性对照质粒瞬时转染至BEL-7402/5-FU细胞;流式细胞术检测细胞凋亡情况;Western blot检测caspase-8,caspase-9和caspase-3蛋白表达水平。结果仅用miR-122处理BEL-7402/5-FU细胞时,与未转染组和转染对照组比较,miRNA-122转染组细胞凋亡率增加,用10μmol/m L 5-FU作用BEL-7402/5-FU细胞24 h后,与未转染组和转染对照组比较,miR-122转染组细胞凋亡率明显增加;与未转染组和转染对照组比较,转染了miRNA-122的BEL-7402/5-FU细胞中caspase-8、caspase-9和caspase-3前体蛋白表达降低,活化的caspase-8、caspase-9和caspase-3蛋白表达升高。结论 miR-122能够下调BEL-7402/5-FU细胞中caspase-8、caspase-9和caspase-3前体蛋白表达,上调活化的caspase-8、caspase-9和caspase-3蛋白表达,miR-122能促进5-FU诱导的BEL-7402/5-FU细胞凋亡。Objective This study deals with the effects of miRNA-122 on BEL-7402/5-FU cell apoptosis induced by 5-fluraeil. Method MiR-122 and control miRNA expression vectors were transient transfected into BEL-7402/5-FU cells. Cell apoptotic percentage was detected by flow cytometry. Western blot was used to detect the caspase-8, caspase-9 and caspase-3 protein expression. Result Flow eytometry results demonstrated that miR-122 transfected cells had a higher apoptosis rate than BEL-7402/5-FU cells or control group. Moreover, miR-122 transfected cells had significant move value than BEL-7402/5-FU cells or control group by 10μmol/ml 5-FU for 24 hours. Western bolt results showed that the expression level of pro-caspase-8, pro-easpase-9 and pro-easpase-3 in miR-122 transfeeted cells were obviously decreased, while the expression level of active easpase-8, caspase-9 and caspase-3 were obviously increased compared with BEL-7402/ 5-FU cells or control group. Conclusion miR-122 down-regnlate pro-caspase-8, pro-caspase-9 and pro-easpase-3 expressions and up-regulate active easpase-8, caspase-9 and caspase-3 expressions in BEL-7402/5-FU cells. MiR-122 increased the cell apoptosis induced by 5-fluracil.

关 键 词：微小RNA-122 BEL-7402/5-FU 5-氟尿嘧啶 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]