阿魏酸对体外低糖低氧损伤兔窦房结细胞凋亡及β-微管蛋白的影响  

Effect of ferulic acid on the apoptosis andβ-tubulin of rabbit sinoatrial node cell in vitro injured by low glucose and hypoxic

作　　者：刘如秀[1] 彭杰[1] 刘宇[1] 刘金凤[1] 汪艳丽[1] 

机构地区：[1]中国中医科学院广安门医院心内科,北京100053

出　　处：《国际中医中药杂志》2015年第4期327-330,共4页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金（81173447）

摘　　要：目的：观察阿魏酸对体外低糖低氧损伤兔窦房结细胞凋亡及β-微管蛋白（β-tubulin）的影响，探讨其作用机制。方法取新生乳兔窦房结细胞，分为空白对照组，模型组，阿魏酸高、中、低剂量组。除空白对照组外，其他组以缺氧缺糖模拟缺血，以恢复氧和糖的供应模拟再灌注造成窦房结细胞损伤模型。在造模更换培养基时，阿魏酸高、中、低剂量组分别加入阿魏酸溶液100、20、10μmol/ml，模型组加入等体积培养基，空白对照组予正常培养基培养。运用流式细胞仪检测各组窦房结细胞凋亡率，激光共聚焦显微镜观察β-tubulin 的形态变化。结果与空白对照组比较，模型组细胞凋亡率[（56.95±11.99）%比（31.45±6.32）%]明显增加（P＜0.01），β-tubulin表达[（5.141±0.218）比（8.035±0.762）]降低；阿魏酸高、中剂量组细胞凋亡率[（24.85±6.34）%、（26.70±9.84）%比（56.95±11.99）%]明显低于模型组（P＜0.01），β-tubulin表达[（7.927±0.357）、（5.961±0.351）比（5.141±0.218）]较模型组升高（P＜0.01）。结论阿魏酸可抑制低糖低氧引起的窦房结细胞凋亡，保护窦房结β-tubulin蛋白形态。Objective To observe the effects of ferulic acid on cell apoptosis andβ-tubulinin vitro of rabbit sinoatrial node injured by hypoxia and low glucose, and to explore its mechanism.Methods Model cells were established on sinoatrial node cells from newborn rabbit by performing deprivation of oxygen and glucose. Then the model cells were divided into 5 groups： a normal control group, a model group, ferulic acid high, medium and low dose group. The normal control group and the model group were treated with equal volume of culture medium, and the three volume ferulic acid groups were treated with ferulic acid of 100, 20, and 10μg/ml respectively. Flow cytometry, laser scanning confocal microscopy were used to observe the sinoatrial node apoptosis rate and cytoskeleton proteinβ-tubulin in each group.Results Apoptosis rate of the model group was obviously higher than the normal group （56.95% ± 11.99%vs. 31.45% ± 6.32%,P〈0.01）, whileβ-tubulin cleavage was significantly lower than the normal group （5.141 ± 0.218vs. 8.035 ± 0.762,P〈0.01）. Apoptosis rate of ferulic acid high, medium dose group were significantly lower than the model group （24.85% ± 6.34%, 26.70% ± 9.84%vs. 56.95% ± 11.99%,P〈0.01）,β-tubulin structure were significantly more complete compared with the model group （7.927 ± 0.357, 5.961 ± 0.351vs. 5.141 ± 0.218,P〈0.01）.Conclusions Ferulic acid can suppress apoptosis of sinoatrial node cells caused by low glucose and oxygen. Protecting the sinoatrial node cell skeleton protein ofβ-tubulin may be one of the mechanisms.

关 键 词：阿魏酸 窦房结细胞 缺血再灌注 细胞凋亡 Β-微管蛋白 

分 类 号：R541.7[医药卫生—心血管疾病]