睾丸肿瘤中β-catenin的表达及其意义  

Expression analysis of β-catenin in testicular cancer

作　　者：邹沙沙[1] 平萍[1] 宋平平[1] 陈婷婷[1] 朱勇[1] 王柱清[1] 刘勇[1] 李铮[1] 胡洪亮[1] 

机构地区：[1]上海交通大学医学院附属仁济医院泌尿外科

出　　处：《中国男科学杂志》2015年第1期9-13,17,共6页Chinese Journal of Andrology

基　　金：国家自然科学基金面上项目(30973069);上海交通大学医学院属仁济医院科研种子基金培养计划(RJZZ14-017)

摘　　要：目的：初步研究β-catenin蛋白在不同类型睾丸肿瘤中的表达特性，并探讨β-catenin分子的表达与睾丸肿瘤的关系，为临床诊断或治疗睾丸肿瘤提供一定依据。方法随机收集我院于2012年1月至2012年12月期间确诊的21例睾丸肿瘤组织和1例梗阻性无精子症（OA）患者的睾丸活检组织（作为正常对照，其睾丸生精功能正常）；同时，选取5只9周龄的ICR小鼠作为实验补充。采用了免疫组化、Western blots 和RT-qPCR等方法来检测β-catenin的表达情况。结果在梗阻性患者中，β-catenin在精原细胞的胞膜和胞浆中呈现低表达；在小鼠中，β-catenin也仅表达在精原细胞的胞浆和胞膜上。在睾丸肿瘤组织中，睾丸孤立性纤维肿瘤中可检测到β-catenin的mRNA和蛋白的高表达，分别为OA患者的9.4倍和17倍，且其β-catenin主要异位表达在细胞核中，可检测到磷酸化的β-catenin的表达。然而，β-catenin在睾丸精原细胞肿瘤和间质细胞肿瘤中不表达或低表达。结论本研究初步表明β-catenin与正常的睾丸生精过程及病理的睾丸孤立性纤维肿瘤发生发展相关，而与睾丸精原细胞肿瘤和间质细胞肿瘤之间无明显相关性。但仍需扩大样本数量进行研究，或采用动物实验深入研究。Objective To study and analyzes the expression pattern and association of β-catenin in testicular cancer, and explore the role of Wnt/β-catenin signaling pathway in the mechanism of testicular cancer occurrence, thus provide evidence for clinical diagnosis and therapy of testicular cancer. Methods We randomly collected 21 cases of testicular cancer biopsy tissue and the testicular biopsy tissue of one obstructive azoospermia patient （OA, as healthy control, due to the completely normal spermatogenic function） in Hospital, from 2012.01 to 2012.12. Five 9w ICR mices were also used in the additional experiments. Expression of β-catenin in the process of spermatogenesis and testicular cancer was detected and quantified by using immunohistochemical, Western blot and RT-qPCR. Results In the obstructive azoospermia patient,β-catenin is weakly expressed in the cell membrane and cytoplasm of spermatogonia. Moreover, in the testis of 9w ICR mices, β-catenin is expressed in the cytoplasm and cell membrane of spermatogonia. However, the results of testicular cancer showed obviously high protein and mRNA expression levels of β-catenin in testicular solitary fibrous tissue, 9.4 folds and 17 folds, respectively, but not in other testicular tumours. β-catenin is ectopicly expressed in the cellular nucleus of testicular solitary fibrous tissue, and we also detected the phosphorylation β-catenin by western blot. Conclusion The results suggested β-catenin may be associated with the process of spermatogenesis and testicular tumor, but not other testicular tumours. However, Considering the limit of sample size, future validating studies in more samples and mechanism study in animal experiments are strongly suggested.

关 键 词：睾丸肿瘤 Β连环素 

分 类 号：R737.21[医药卫生—肿瘤]