DNA聚合酶Iota(Polι)与食管鳞癌淋巴结转移的相关性研究  

Correlation analysis between DNA polymerase Iota(Polι) and lymph node metastasis in human esophageal squamous carcinoma

作　　者：孙昊尧[1] 邹士涛[1] 周俊东[1] 朱孝中[2] 刘标[1] 孟星君[1] 李晓庆[1] 虞健[1] 吴锦昌[1] 

机构地区：[1]南京医科大学附属苏州医院疗放科,江苏苏州215001 [2]江苏大学附属江滨医院胸外科,江苏镇江212001

出　　处：《南京医科大学学报（自然科学版）》2015年第3期304-308,319,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81372433);江苏省自然科学基金(BK20131149);苏州市科技局应用基础研究计划(SYS201360)

摘　　要：目的 :探讨DNA聚合酶Iota(Polι)与食管鳞癌淋巴结转移的关系。方法 :采用实时荧光定量PCR方法检测Polι基因在食管鳞癌组织中的表达,并使用Mann-Whitney U检验方法分析Polι与食管鳞癌淋巴结转移之间的关系;利用免疫组织化学染色法检测Polι及Nm23蛋白在食管鳞癌组织中的表达,并使用Spearman相关分析研究食管鳞癌中Polι与Nm23表达的相关性。向食管癌细胞TE-1和ECA-109转染Polι表达载体,上调细胞中Polι的表达,Realtime-PCR检测Polι和Nm23 m RNA的表达水平,并通过Transwell侵袭实验分析细胞的侵袭能力。结果:在食管鳞癌临床组织标本中,Polι表达与淋巴结转移相关(P<0.01),与Nm23蛋白表达呈负相关(R=-0.481,P<0.05)。上调Polι的表达增强了TE-1和ECA-109细胞的侵袭能力,差异有统计学意义(P均<0.05),同时抑制了细胞中Nm23 m RNA的表达,差异有统计学意义(P<0.001)。结论:Polι的表达与食管鳞癌的转移密切相关,其可能通过下调Nm23的表达来促进肿瘤转移。Objective：To investigate relationship between DNA polymerase iota （PolL） and lymph node metastasis of human esophageal squamous cell carcinoma. Methods：PolL expression in esophageal squamous cell carcinoma tissues was detected by Real- time PCR. Mann-Whitney U test was performed to analyze the relationship between Poll and lymph node metastasis. Poll and Nm23 expression in esophageal squamous cell carcinoma tissues was detected by immumohistochemieal staining. Spearman correlation analysis was performed to analyze the relationship between Poll and Nm23. The Poll expression vector was transfected into esophageal cancer cells TE-1 and ECA-109 to up-regulate Poll expression. Poll and Nm23 mRNA expression was detected by Reahime-PCR. Transwell chamber assay was performed to analyze cell invasional ability. Results：In clinical tissue samples ,Poll expression was positively correlated with the lymph node metastasis of human esophageal squamous cell carcinoma（P 〈 0.01）,and negatively correlated with Nm23 in protein level（R=-0.481 ,P 〈 0.05）. In vitro study,overexpression of Poll enhanced cell invasion（P 〈 0.05） and significantly inhibited Nm23 mRNA expression in TE-1 and ECA-109 cells （P 〈 0.001 ）. Conclusion： Poll is closely related with the invasion and metastasis of esophageal squamous cell carcinoma,the possible mechanism of the regulation is through down regulating the expression of Nm23.

关 键 词：Polι NM23 肿瘤转移 食管鳞癌 

分 类 号：R735.1[医药卫生—肿瘤]