乙醇脱氢酶2基因多态性与脑梗死的关系  

Association between alcohol dehydrogenase 2 genetic polymorphisms and cerebral infarction

作　　者：许海东[1,2] 王敏 耿德勤[4] 

机构地区：[1]徐州医学院研究生院,江苏徐州221004 [2]滕州市中心人民医院神经内科,山东滕州277500 [3]山亭区人民医院脑电图室,山东枣庄277200 [4]徐州医学院附属医院神经内科,江苏徐州221002

出　　处：《临床荟萃》2015年第4期379-382,共4页Clinical Focus

摘　　要：目的探讨乙醇脱氢酶2(ADH2)基因多态性与脑梗死的关系。方法应用病例对照研究的方法,选择滕州市中心人民医院神经内科就诊的198例脑梗死患者为脑梗死组,同时选择200例与脑梗死组性别、年龄相匹配的健康体检者为对照组。收集研究对象一般临床资料并采集静脉血,采用聚合酶链反应结合限制性片段长度多态性(PCR-RFLP)方法测定两组研究对象ADH2基因型并计算等位基因频率。采用Logistic回归分析方法分析脑梗死与ADH2基因型之间的关系。结果脑梗死组ADH2*1/*1基因型的频率分布高于对照组(11.6%vs 5.0%,P<0.05);脑梗死组ADH2*1等位基因的频率分布亦高于对照组(30.8%vs 21.0%,P<0.05)。多因素logistic回归分析结果显示,ADH2*1/*1基因型者患脑梗死的风险是非ADH2*1/*1基因型者的17.70倍(P<0.05)。结论 ADH2*1/*1基因型可能是脑梗死发生的危险因素,可以为进一步研究脑梗死发生的生物标志物提供病因线索。Objective To investigate the relationship of alcohol dehydrogenase 2（ADH2） genetic polymorphisms with cerebral infarction.Methods A case-control study was conducted on 198 patients with cerebral infarction（CI）selected from Neurology Department of Tengzhou Central People＇s Hospital as CI group,and 200 healthy examination people from health examination center were selected as control group matched with the CI group in gender and age.The clinical data and venous blood were collected from CI group and control group.The ADH2 genotype was detected by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）method and allele frequencies were calculated.Logistic regression analysis was used to analyze the association between the ADH2 genotype and CI.Results ADH2＊1/＊1genotype distribution of CI group（11.6%）was higher than that of control group（5.0%）（P 〈0.05）.ADH2＊1allele frequency of the CI group（30.8%）was higher than that of control group（21.0%）（P〈 0.05）.Multi-factor logistic regression analysis showed that the risk of ADH2＊1/＊1genotype suffered from cerebral infarction was 17.70 times over that without ADH2＊1/＊1genotype（P〈 0.05）.Conclusion ADH2＊1/＊1genotype is likely to be a risk factor of CI.And the ADH2＊1/＊1genotype may be used as a biomarker to predict the risk of CI.

关 键 词：脑梗死 染色体结构变异 乙醇脱氢酶 2 多态性 

分 类 号：R743[医药卫生—神经病学与精神病学]