全反式维甲酸联合低剂量阿糖胞苷诱导人急性粒-单核白血病细胞株ACC247凋亡的研究  

Differentiation and apoptosis of acute myelomonocytic leukemia cell line ACC247 induced by combination of ATRA and low dose Ara-C

作　　者：刘召鑫[1] 任秀玉[1] 徐鑫[1] 赵瑶[1] 胡振波[1] 

机构地区：[1]潍坊医学院,山东潍坊261041

出　　处：《临床血液学杂志》2015年第2期201-203,208,共4页Journal of Clinical Hematology

基　　金：国家自然科学基金(No:81370628)

摘　　要：目的:以急性粒-单核白血病细胞株ACC247为模型,采用全反式维甲酸(ATRA)、低剂量阿糖胞苷(Ara-C)单药及二者联合应用,观察其对ACC247细胞凋亡的影响。方法:用ATRA、低剂量Ara-C单药及二者联合处理ACC247细胞48h后,分别用MTT法检测细胞增殖状况,Annexin V-FITC/PI双染检测细胞凋亡,倒置显微镜观察细胞形态判断分化。结果:单独ATRA作用于ACC247细胞,随着作用时间的增加可见细胞体积膨胀,细胞核固缩,抑制其增殖并促进其向成熟分化,进而凋亡,呈时间依赖性,剂量固定为1μmol/L。单独低剂量Ara-C作用于ACC247白血病细胞,随着作用时间的增加可以使细胞数量减少,部分细胞核碎裂,细胞裂解,抑制其增殖并促进其凋亡,呈时间依赖性。ATRA和低剂量Ara-C二者联合应用,对ACC247细胞增殖抑制与诱导凋亡作用增强。结论:ATRA联合低剂量Ara-C可以抑制ACC247细胞增殖,促进其凋亡;联合用药的效果优于单用ATRA或Ara-C。Objectives：To explore the effects of all-trans retinoid acid （ATRA） and low dose cytosine arabinoside （Ara-C）,either individually or in combination on the proliferation, apoptosis and differentiation of acute myelomonocytic leukemia cell line ACC247 cells. Methods：ACC247 cells were treated with ATRA and low dose Ara-C,either individually or in combination for different time. Cell proliferation was studied by MTT assay. Flow cytometry analysis of Annexin V-FITC/PI dual staining was used to examine apoptosis. Cell morphology was observed under the light microscope. Resuits：ATRA inhibited the proliferation and promoted differentiation of ACC247 cells in a time- dependent manner. Treatment of ACC247 cells by low dose Ara-C inhibited proliferation and induced apoptosis in a time-dependent manner. The combination of ATRA and Ara-C resulted in differentiation and apoptosis of ACC247 cells. Conclusion .. Combinationof ATRA and low dose Ara-C can inhibit proliferation,induce differentiation and ap- optosis of ACC247 ceils. The data suggests that combination usage of ATRA and low dose Ara-C may be a useful treatment for acute myelomonocytic leukemia（M4）.

关 键 词：全反式维甲酸 阿糖胞苷 ACC247细胞 增殖 凋亡 

分 类 号：R733.71[医药卫生—肿瘤]