高效液相色谱-内标法测定焦性没食子酸含量（英文）  

Determination of Pyrogallol Using Reversed-phase HPLC and Internal Standard Method

作　　者：张亮亮[1,2,3,4] 汪咏梅[1,2,3,4] 徐曼[1,2,3,4] 陈笳鸿[1,2,3,4] 

机构地区：[1]中国林业科学研究院林产化学工业研究所 [2]生物质化学利用国家工程实验室 [3]国家林业局林产化学工程重点开放性实验室 [4]江苏省生物质能源与材料重点实验室,江苏南京210042

出　　处：《生物质化学工程》2015年第2期12-16,共5页Biomass Chemical Engineering

基　　金：国家863计划资助(2014AA021802)

摘　　要：建立了一种方便、快速、准确的测定焦性没食子酸含量的高效液相色谱-内标分析方法。色谱条件为：Thermo HypersilC18（250mm＆#215;4.6mm,5μm）色谱柱,流动相为甲醇/水（含0.1%的三氟乙酸）,梯度洗脱,流速为1.0mL/min,紫外检测波长为266 nm。选择鞣花酸作为内标物。结果表明在该色谱条件下,焦性没食子酸和鞣花酸的保留时间分别为4.33和19.25 min,焦性没食子酸质量浓度在62.5~2000 mg/L范围内呈良好线性关系,线性方程y=0.00013866x＋0.00192（R=0.9997）,平均加样回收率为103.6%。该方法简便、准确、重现性好,可适用于焦性没食子酸产品含量的分析测定。A rapid, specific, and accurate HPLC method was developed and validated for the assay of pyrogallol. The assay involved a gradient elution of pyrogallol with a Thermo Hypersil C18 column （250 mm ＆#215; 4. 6 mm,5 μm） using mobile phase composition of methanol and water （0. 1% TFA） at a flow rate of 1. 0 mL/min. UV detection was performed at 266 nm. Ellagic acid was used as an internal standard. The retention time of pyrogallol and ellagic acid was 4. 33 and 19. 25 min,respectively. The linearity range of pyrogallol concentration was from 62. 5 to 2 000 mg/L. The fitting equation of calibration curve was y =0. 000 138 66x＋0. 001 92 （R=0. 999 7） and a mean recovery of 103. 6% was found. The proposed method was applied for the quantitative determination of pyrogallol in pyrogallol products.

关 键 词：焦性没食子酸 没食子酸 反相高效液相色谱法 内标法 

分 类 号：O657.72[理学—分析化学] TQ241[理学—化学]