胃癌细胞herg mRNA、HERG蛋白表达及HERG电流强度变化  

Expression of herg gene,protein and changes of HERG current in gastric cancer cells

作　　者：邵晓冬[1] 张永国[1] 陈江[1] 王金玲[1] 郭晓钟[1] 任丽楠[1] 

机构地区：[1]沈阳军区总医院,沈阳110016

出　　处：《山东医药》2015年第2期1-4,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30400204)

摘　　要：目的探讨胃癌细胞herg mRNA、HERG蛋白表达及HERG电流强度的变化的临床意义。方法培养胃癌细胞(胃癌细胞系SGC7901、MGC803、AGS、MKN45)及永生化胃上皮细胞(GES),取对数生长期细胞用于实验。采用RT-PCR法检测herg mRNA表达,Western blot法检测HERG蛋白表达,采用全细胞膜片钳技术测定SGC7901及GES的HERG电流强度。结果 herg mRNA及其蛋白在4种胃癌细胞系中均有表达,AGS中HERG蛋白表达量低于其他三种细胞系(P均<0.05);GES中无herg mRNA及其蛋白表达。在SGC7901中检测到HERG电流,GES中未记录到HERG电流。结论 herg mRNA及其蛋白在胃癌细胞系SGC7901、MGC803、AGS、MKN45表达增高,SGC7901中存在HERG电流。HERG蛋白可能参与了胃癌的发生,并与胃癌恶性程度有关。Objective To investigate the expression of herg gene, HERG protein and HERG current in gastric cancer cells. Methods Gastric cancer cells and gastric epithelial （GES） cells were cultured to logarithmic phase. The expres- sions of berg mRNA and HERG protein in gastric cancer cells and GES cells were measured by using RT-PCR and Western blot, respectively. The whole cell configuration of the patch-clamp technique was employed to record HERG currents in va- rious cells. Results HERG mRNA and protein were positively expressed in four gastric cancer cell lines. Expression level of HERG protein in AGS cells was lower compared with the other three gastric cancer cell lines （P 〈 0.05 ）. There was negative expression of berg mRNA and HERG protein in GES cell line. HERG current was detected in gastric cancer cell, whereas there was no HERG current in GES cell. Conclusion Herg gene and HERG protein were highly expressed in gastric cancer cells and HERG current was exclusively detected in gastric cancer cells. HERG protein was associated with carcinogenesis of gastric cancer and may serve as a diagnostic marker for gastric cancer.

关 键 词：胃肿瘤 胃癌 HERG基因 HERG蛋白 HERG电流 延迟整流钾通道 肿瘤形成过程 

分 类 号：R735.2[医药卫生—肿瘤]