QuEChERS-液相色谱法同时测定蜂蜜中6种氟喹诺酮类药物的残留  

Simultaneous Determination of Six Kinds of Fluoroquinolones Residuals in Honey by QuEChERS-HPLC

作　　者：杨微微[1] 杨勇[2] 罗曼[3] 许乾丽[3] 罗奕[1] 王俪瓯 

机构地区：[1]贵阳医学院,贵州贵阳550005 [2]贵阳中医学院,贵州贵阳550002 [3]贵州省食品药品检验所,贵州贵阳550004

出　　处：《贵州农业科学》2015年第2期165-169,共5页Guizhou Agricultural Sciences

基　　金：贵州省科技计划项目"贵州动物源性食品氟喹诺酮类药残留及快速筛查技术研究"[黔科合SY字(2012)3061]

摘　　要：为建立一种简便、快速、重现性好的高效液相色谱-荧光检测器检测蜂蜜中的诺氟沙星、环丙沙星、单诺沙星、恩诺沙星、沙拉沙星、双氟沙星6种氟喹诺酮残留量的方法,采用QuEChERS-液相色谱法同时测定蜂蜜中6种氟喹诺酮类药物残留。结果表明：QuEChERS测定6种氟喹诺酮的分离度较好,在0.02~0.5μg/mL具有良好的线性关系（r=0.999 9）,检出限为0.97~3.91μg/kg,平均加标回收率为72%~115%。相对标准偏差在1.4%~11%。To establish a method for determining six kinds of fluoroquinolones （Norfloxacin, Ciprofloxacin,Danofloxacin,Enrofloxacin,Sarafioxacin,Difloxacin）in honey with simple pretreatment, high sensitivity and good repeatability.Simultaneous determination of the six kinds of fluoroquinolones residuals was conducted by QuEChERS-HPLC. Results： The calibration curves of six kinds of fluoroquinolones showed good linearities in the range of 0.02~0.5 μg/mL（r =0.999 9）.The detection ranged from 0.97 to 3.91 μg/kg.The mean recoveries at the levels of samples was 72% ~ 115%.The relative standard deviations was between 1.4%~11%.

关 键 词：HPLC-FLD 蜂蜜 氟喹诺酮 药物残留 

分 类 号：S896.8[农业科学—特种经济动物饲养]