微乳体系相图及其在液相色谱分离多组分药物应用的研究  

Phase Diagram of Microemulsion System and Its Application in Separation of Multi-component Drug by Liquid Chromatography

作　　者：陈嘉敏[1] 冯晓华[1] 潘晓玲[1] 黄丽娜[1] 高崇凯[1] 李宁[1] 

机构地区：[1]广东药学院药科学院,广东广州510006

出　　处：《分析测试学报》2015年第4期433-437,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金(81173525)

摘　　要：采用滴加法和电导率法绘制了十二烷基硫酸钠(SDS)/正丁醇/油相(正辛醇、正辛烷、正庚烷、正己烷)/水四组分微乳体系的拟三元相图。以O/W型微乳区域大小为指标,考察不同油相、表面活性剂与助表面活性剂的质量比对微乳形成的影响,并通过测定电导率、相对粘度以及表面张力等物理化学参数,考察了微乳体系的相行为及特性。根据得到的微乳体系相图,进行色谱条件优化,建立了快速、稳定的测定阿莫西林舒巴坦匹酯片剂中阿莫西林与舒巴坦匹酯二组分含量的方法,结果显示此微乳分离系统有较好的色谱适用性及方法可行性。The quasi-ternary phase diagrams for microemulsion systems containing sodium dodecyl sulfate（ SDS）,n-butanol,oil phase（ n-octanol,n-octane,n-heptane,n-hexane） and water were plotted by the titration and electrical conductivity. Effects of oil type,mass proportion of surfactant and co-surfactant upon the forming of O / W microemulsion were investigated in terms of the region for O / W microemulsion in the phase diagram. Physical and chemical parameters including electrical conductivity,interfacial tension and viscosity were used to elucidate the phase behaviors and characteristics of microemulsion systems. According to the microemulsion phase diagram,the chromatographic conditions were optimized. A rapid and robust separation method was developed and validated for the determination of Amoxicillin- Sulbactam Pivoxil tablets. Good suitability and separation selectivity of HPLC were obtained.

关 键 词：微乳液相色谱 拟三元相图 十二烷基硫酸钠 阿莫西林舒巴坦匹酯片 

分 类 号：O657.7[理学—分析化学] R917[理学—化学]