间接竞争酶联适配体检测食品中土霉素  

Determination of Oxytetracycline in Food by Indirect Competitive Enzyme-linked Aptamer Assay

作　　者：唐宗贵[1] 刘长彬[2] 罗小玲[1] 康立超[1] 孙凤霞[1] 卢春霞[1] 

机构地区：[1]新疆农垦科学院分析测试中心农业部食品质量监督检验测试中心(石河子),新疆石河子832000 [2]新疆农垦科学院畜牧兽医研究所,新疆石河子832000

出　　处：《分析测试学报》2015年第4期458-462,467,共6页Journal of Instrumental Analysis

基　　金：新疆农垦科学院科技引导计划(62YYD201309);国家科技支撑计划(2012BAD43B03)

摘　　要：研究建立了一种间接竞争酶联适配体检测食品中土霉素（OTC）的分析方法。通过方阵滴定法和单因素实验优化了检测条件。在最佳实验条件下,方法的半抑制浓度（IC50）为6.3 ng/m L,对OTC的检测线性范围为0.5-50 ng/m L;与结构类似物有较低的交叉反应;对牛奶、奶粉、鸡肉、水产品和蜂蜜样品中土霉素的加标回收率为62.1%-102%,相对标准偏差（RSD）小于15%。将建立的方法用于实际样品检测,并与国标方法进行对比,两者获得较高的相关性（r^2=0.979）。本方法可实现对实际样品中土霉素的快速、高灵敏和高通量检测。An indirect competitive enzyme-linked aptamer assay（ ic- ELAA） was developed for monitoring oxytetracycline（ OTC） in complex food matrix. Various factors such as OTC- BSA concentration,aptamer concentration,avidin- HRP concentration, coating buffer, blocking agent, and binding buffer were evaluated for the performance of ELAA. The optimized ELAA showed a good sensitivity（ IC50 of 6. 3 ng / m L） and a wide linear range（0. 5- 50 ng / m L） for OTC. There was no obvious cross reactivity（ CR） with most of the tetracyclines pesticides. Recoveries of OTC in food samples,including milk,milk powder,chicken,fish and honey,ranged from 62. 1% to 102% with relative standard deviations（ RSDs） less than 15%. In addition,the developed ELAA showed a good correlation with the high performance liquid chromatographic method（ r^2= 0. 979）. The results indicated that the developed method was a simple,sensitive,accurate and cost-effective approach for the screening of OTC in food samples.

关 键 词：土霉素 适配体 酶联适配体 食品 

分 类 号：O657.63[理学—分析化学] O625.32[理学—化学]