MiR-17-3P对多发性骨髓瘤细胞增殖的影响  

The effect of miR-17-3P on multiple myeloma cells proliferation

作　　者：王顺业 徐瑞容[1] 黄红铭[1] 丁润生[1] 那文秀 张立娜[1] 

机构地区：[1]南通大学附属医院血液科,江苏226001

出　　处：《交通医学》2015年第1期4-6,共3页Medical Journal of Communications

基　　金：江苏省自然科学基金资助项目(BK2011388);南通大学研究生创新课题计划(YKC13082)

摘　　要：目的:探究miR-17-3P对多发性骨髓瘤(MM)细胞增殖的影响。方法:通过实时荧光定量聚合酶链反应测定miR-17-3P在MM细胞系RPMI 8226、U266和KM3及正常人骨髓细胞中的表达情况。合成miR-17-3P模拟物和抑制物,分别转染U266细胞,采用MTT法测细胞活力,PI染色流式细胞仪测细胞周期,观察U266细胞增殖情况。结果:MM细胞株RPMI8226、U266、KM3中miR-17-3P相对表达量均明显高于正常人骨髓细胞;利用模拟物上调miR-17-3P后,U266的细胞活力较对照组明显提高,48h时作用效果最为显著;相反,下调miR-17-3P后,U266的细胞活力降低;上调miR-17-3P后,G1期细胞比例较对照组有所减少,S期细胞比例明显增多;相反,下调miR-17-3P后,细胞多被阻滞在G1期。结论:Mi R-17-3P可能促进多发性骨髓瘤细胞增殖。Objective：To study the effection of miR-17-3P on cell proliferation of multiple myeloma. Methods：The expressions of miR-17-3P in MM cell lines RPMI8226,U266,KM3 and the healthy controls were measured by real-time PCR. After the construction of the angomiR-17-3P and antagomiR-17-3P, they were transfected into U266, the cell proliferation was measured by MTT, growth curve was drawn, and then the cell cycle was measured with PI stained by flow cytometry. Results： AngomiR-17-3P mimics could increase the expression of corresponding miRNA, promote the proliferation of MM cells; AntagomiR-17-3P inhibitor could reduce the expression of corresponding miRNA and suppress the proliferation of MM cells. Conclusion：MiR-17-3P could promote the cell proliferation of multiple myeloma.

关 键 词：多发性骨髓瘤 U266细胞 实时荧光定量聚合酶链反应 流式细胞仪检测 MTT比色法 

分 类 号：R733.3[医药卫生—肿瘤]