晚期肺癌患者γδT细胞CD27和CD28表达检测的临床应用价值  

The clinical value of analyzing expressions of CD27 and CD28 in γδT lymphocytes in patients with advanced lung cancer

作　　者：郭丽[1] 莫娟芬 阮玲娟[1] 王安涛[1] 钟征翔[1] 王兆丰[1] 鲍轶[1] 

机构地区：[1]浙江省嘉兴市第二医院中心实验室,314000

出　　处：《中华全科医学》2015年第5期694-696,共3页Chinese Journal of General Practice

基　　金：国家自然科学基金青年科学基金项目(811010707);浙江省中医药基金(2014ZB119);浙江省嘉兴市科技计划项目(2012AY1071-2)

摘　　要：目的分析晚期肺癌患者外周血及胸腔积液中γδT细胞CD27和CD28的表达,并与健康正常人对照组进行比较,探讨其分化表型及临床应用价值。方法收集32例晚期肺癌患者外周血和其中伴恶性胸腔积液患者的17例积液标本。选取健康对照组22例,按照年龄分30岁以下青年组和50岁以上中老年组。利用流式细胞仪检测标本γδT细胞表面CD27和CD28分子的表达。比较不同年龄组的健康人,晚期肺癌患者与健康对照组的外周血,以及肺癌患者外周血与胸腔积液间的CD27及CD28在γδT细胞上的表达差异。结果健康人组中,中老年组与青年组相比,γδT细胞的比例差异无统计学意义(P>0.05),但是中老年组的CD27/CD28双阳性细胞占γδT细胞比例明显降低(P<0.05),双阴性细胞比例显著降低(P<0.01)。晚期肺癌患者的外周血与同年龄组的健康人相比,γδT细胞的比例显著降低(P<0.01),其中CD27/CD28双阳性细胞占γδT细胞比例与健康人组相比差异无统计学意义(P>0.05),但是晚期肺癌患者的双阴性细胞比例显著性升高(P<0.01)。晚期肺癌患者外周血和胸腔积液中的γδT细胞上4种CD27和CD28分化表型比例之间差异无统计学意义(P>0.05)。结论晚期肺癌患者γδT细胞及其上的CD27和CD28的分化表型可反映其免疫功能,可为肺癌治疗及估计预后提供依据。Objective To explore the different expressions of CD27 and CD28 in γδT lymphocytes of peripheral blood and malignant pleural effusion in patients with advanced lung cancer and healthy donors. Methods A total of 32 clinical blood samples and 17 malignant pleural effusion samples were collected from patients diagnosed with advanced lung cancer. Peripheral blood was obtained from 22 healthy donors,they were divided into two groups,the young group under the age of 30 and older than 50 in another group. Flow cytometry was employed to detect the expressions of CD27 and CD28 on the surfaces of γδT lymphocytes. The different expression between healthy groups,advanced lung cancer patients and healthy controls,peripheral blood and malignant pleural effusion in patients with advanced lung cancer were compared. Results The percentage of γδT cells was not different between two healthy donor groups. The frequency of γδT ＋ CD27＋CD28＋T cells and γδT ＋ CD27-CD28-T cells was significantly lower in older-donors than younger-donors（ P〈0. 05 and P〈0. 01,respectively）. The percentage of γδT cells was significantly lower in patients with advanced lung cancer than in age matched healthy donors. No difference was observed upon the frequency of γδT^＋ CD27^＋CD28^＋T cells between patients with lung cancer and healthy controls,but the frequency of γδT ^＋ CD27^-CD28^-T cells was significantly higher in patients（ P〈0. 01）. No difference was observed upon the frequency of CD27 and CD28 on the γδT cells between the peripheral blood and matched malignant pleural effusion in patients with advanced lung cancer. Conclusion The different phenotype of CD27 and CD28 of γδT lymphocytes in patients with lung cancer can reflect their immune activates,it can provide evidence for monitoring treatment and prognosis of lung cancer.

关 键 词：肺癌 恶性胸腔积液 ΓΔT细胞 CD27 CD28 

分 类 号：R734.2[医药卫生—肿瘤] R561.3[医药卫生—临床医学]