富贵籽茎段诱导腋芽增殖与试管苗培育研究  

The Experimental Studies on Induced Growth and Proliferation of Axillary Buds of Different Stem Segments and Plantlet Culture of Ardisia Ccrenata Sims

作　　者：孔祥海[1] 邱丰艳[1] 丁力[1] 陈小红[1] 

机构地区：[1]龙岩学院,福建龙岩364000

出　　处：《龙岩学院学报》2015年第2期89-93,共5页Journal of Longyan University

摘　　要：以不同植物激素组合和不同发育阶段的茎段为实验因子,开展富贵籽茎段诱导腋芽生长及其增殖和试管苗培育的实验研究。结果:(1)MS+NAA 0.1 mg·L-1+BA 0.50 mg·L-1的培养基配方较适合于从富贵籽无菌苗和当年萌发的嫩枝茎段诱导腋芽生长与增殖;(2)MS+NAA 0.1 mg·L-1+BA 1.50 mg·L-1的组合配方能较好的诱导富贵籽1年生茎段腋芽生长与增殖。(3)1/2MS+NAA 0.50 mg·L-1+IBA 0.30 mg·L-11的组合配方用于不定芽体诱导生根和培育试管苗的效果最好。Ardisia crenata is important ornamental and medicinal plants. The experimental effect research of the different combinations of plant hormones on induced growth and proliferation axillary buds of aseptic stems, yearly germinating shoots stems and one-year-old stem of perennial plants were carried out. The results were as follows ： The medium formula of MS ＋ NAA 0.1 mg · L^-1（the same as below） ＋ BA 0.50 was suitable for induced growth and proliferation axillary buds of aseptic stems and yearly germinating shoots stems, while the medium formula of MS ＋ NAA 0.10 ＋ BA 1.50 was suitable for induced growth and proliferation axillary buds of 1-year old stems. The combination formula of 1/2 MS ＋ NAA 0.50 ＋IBA 0.30 had best effect for adventitious bud rooting and the cultivation of test tube plantlets.

关 键 词：富贵籽 茎段 腋芽 诱导 增殖 试管苗 

分 类 号：Q943.1[生物学—植物学]