白藜芦醇对CXCL12诱导的胃癌细胞增殖的影响  

Effects of Resveratrol on the CXCL12-induced Proliferation of Gastric Cancer Cells

作　　者：王玲琳[1] 朱妍[1] 朴花子[1] 

机构地区：[1]延边大学医学院药理学教研室,吉林延吉133002

出　　处：《肿瘤药学》2015年第2期101-105,共5页Anti-Tumor Pharmacy

基　　金：吉林省自然科学基金(20125236)

摘　　要：目的探讨白藜芦醇对趋化因子CXCL12诱导的人胃癌SGC-7901细胞增殖及其CXCR4、CXCR7和VEGF m RNA表达的影响。方法用CXCL12刺激人胃癌SGC-7901细胞后,采用MTT法检测细胞增殖率;应用RT-PCR法检测细胞内CXCR4、CXCR7和VEGF m RNA的表达水平。同时观察白藜芦醇对CXCL12所诱导的人胃癌SGC-7901细胞增殖率,CXCR4、CXCR7和VEGF m RNA表达水平的影响。结果不同浓度的CXCL12均可以显著促进人胃癌SGC-7901细胞体外增殖(P<0.001)。CXCL12可显著上调SGC-7901细胞CXCR4、CXCR7和VEGF的m RNA表达,而白藜芦醇显著抑制CXCL12所诱导的胃癌细胞体外增殖和CXCR4、CXCR7、VEGF m RNA的表达。结论白藜芦醇抑制CXCL12诱导的胃癌细胞增殖,其机制可能与下调CXCR4和CXCR7水平,从而抑制VEGF分泌有关。Objective To study the effects of resveratrol on the CXCL12-induced proliferation of gastric cancer cell line SGC-7901 and m RNA expression of CXCR4, CXCR7 and VEGF. Methods SGC-7901 cells were treated with CXCL12 and resveratrol, then the proliferation of the SGC-7901 cells were tested by MTT. The levels of CXCR4, CXCR7 and VEGF m RNA were measured by reverse transcription-polymerase chain reaction（RT-PCR）. Results Different concentrations of CXCL12 all significantly increased the in vitro proliferation of SGC-7901 cells（P〈0.001）. CXCL12 also up-regulated the m RNA expressions of CXCR4, CXCR7 and VEGF in SGC-7901 cells. We found that resveratrol significantly inhibited CXCL12-induced proliferation of SGC-7901 cells and m RNA expressions of CXCR4, CXCR7 and VEGF. Conclusion Resveratrol could inhibit CXCL12-induced proliferation of gastric cancer cells. The mechanism may be related with the down-regulation of CXCR4, CXCR7 and VEGF levels.

关 键 词：CXCL12 细胞增殖 VEGF CXCR4 CXCR7 白藜芦醇 

分 类 号：R735.2[医药卫生—肿瘤]