结肠癌细胞上皮-间充质转化后β1整合素表达变化及意义  

The effect of epithelial-mesenchymal transition of human colon carcinoma on intergin-β1 expression and significance

作　　者：纪奕顺 高军[1] 高品[1] 吕春龙[1] 郑淑龙 

机构地区：[1]青岛大学附属医院普外科,266003

出　　处：《中华实验外科杂志》2015年第4期708-710,共3页Chinese Journal of Experimental Surgery

基　　金：山东省自然科学基金资助项目

摘　　要：目的 观察SW480结肠癌细胞上皮-间充质转化（EMT）后β1整合素表达变化,探讨其对结肠癌细胞体外侵袭力的影响.方法 用10 μg/L转化生长因子-β1（TGF-β1）处理72 h后检测SW480结肠癌细胞发生EMT.实时定量聚合酶链反应（Real-time PCR）及Western blot方法分别检测转化前后癌细胞β1整合素mRNA和蛋白的表达;体外侵袭实验检测转化前后SW480细胞的体外侵袭能力.结果 EMT后结肠癌细胞β1整合素mRNA表达较转化前明显增强（t=3.764,P＜0.05）;EMT后结肠癌细胞β1整合素蛋白表达（0.430±0.021）较转化前（0.172 ±0.034）明显增强（P＜ 0.05）;Transwell细胞侵袭实验显示EMT后结肠癌细胞的穿膜细胞数[（137±9）个]较转化前[（36±4）个]明显增多（P＜0.05）.结论 结肠癌细胞EMT后β1整合素表达升高,其体外侵袭力增强.Objective To investigate the expression of intergin-β1 of human colon carcinoma cell line SW480 after epithelial-mesenchymal transition （EMT） and effect of invasive ability on the human colon carcinoma in vitro.Methods The epithelial-mesenchymal transition of SW480 cells was introduced by transforming growth factor-β1 （TGF-β1,10 μg/L） for 72 h.The expression of intergin-β1 in SW480 cells was detected by real-time quantitative polymerase chain reaction （Real-time PCR） and Western blotting.The migration of SW480 cells in vitro was assayed by Transwell.Results In contrast with that before EMT,the expression of intergin-β1 mRNA in SW480 cells after EMT was increased obviously （t =3.764,P 〈 0.05）.As compared with that before EMT （0.172 ± 0.034）,the expression of intergin-β1 protein in SW480 cells after EMT （0.430 ±0.021） was increased obviously （P 〈0.05）.The migration of SW480 cells after EMT （137 ± 9） was significantly increased （P 〈 0.05） as compared with that before EMT （36 ±4）.Conclusion The expression of intergin-β1 and the migration of human colon carcinoma cells in vitro can be elevated after EMT.

关 键 词：结肠癌 上皮-间充质转化 Β1整合素 Intergin-β1 

分 类 号：R735.35[医药卫生—肿瘤]