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作 者:余鹏飞[1] 白槟[1] 徐斌[1] 郝一鸣[1] 邱兆岩 王谦[1] 冯全新[2] 赵青川[3]
机构地区:[1] 第四军医大学西京消化病医院肿瘤生物学国家重点实验室,西安710032 [2] 第四军医大学西京消化病医院消化监护室,西安710032 [3] 第四军医大学西京消化病医院消化外科,西安710032
出 处:《中华实验外科杂志》2015年第4期764-766,共3页Chinese Journal of Experimental Surgery
基 金:国家自然科学基金资助项目(81370564)
摘 要:目的 观察不同剂量雨蛙素小鼠腹腔注射时其胰腺炎严重程度的变化.方法 采用C57小鼠以雨蛙素50 μg/kg分别间断腹腔注射3、6、9、12次诱发胰腺炎,检测血清淀粉酶和脂肪酶、胰腺水含量、胰腺组织苏木素-伊红(HE)染色及组织学病理评分、肺脏组织HE染色.结果 常规雨蛙素剂量(50 μg/kg)分别注射3、6、9、12次时,实验组血清淀粉酶明显高于对照组(P<0.05),在9次时达到高峰[(25 966.67±3 787.02) U/L];胰腺水含量在实验组中显著高于对照组(P<0.05),注射6次时达到峰值[(81.13±1.03)%];胰腺组织HE染色结果显示注射3次胰腺HE染色仅有轻度水肿和少量炎细胞浸润,6次时可见大量中性粒细胞浸润,明显水肿,9次时胰腺小叶边缘出现少量的坏死灶,12次可见到大范围的腺泡坏死灶[组织病理评分(8.20±0.84)分],镜下表现出坏死性胰腺炎改变.结论 在C57小鼠体内,常规雨蛙素剂量(50 μg/kg)间断腹腔注射6次可诱发出急性水肿型胰腺炎,而12次可诱发出坏死性胰腺炎改变.Objective To explore the severity of acute pancreatitis induced by intraperitoneal injection of 50 μg/kg caerulein at different doses in mice.Methods Acute pancreatitis (AP) was induced by intraperitoneal infection of 50 μg/kg caerulein at 3,6,9,12times,respectively.We evaluated the severity of AP biochemically and morphologically.Results Conventional doses of caerulein (50 μg/kg) were injected with 3,6,9,12 times,the level of serum amylase in experimental group were significantly higher than that in control group (P 〈 0.05),peaked in the 9 times [(25 966.67 ± 3 787.02) U/L] ;pancreatic water content in the experimental group was significantly higher than that in control group (P 〈 0.05),peaked in the 6 times [(81.13 ± 1.03) %] ; pancreatic tissue hematoxylin and eosin (HE) staining showed that pancreas was slightly edema and infiltration of inflammatory cells at 3 times,which showed a large amount of neutrophil infiltration and obvious edema at the 6 times,and pancreatic leaflet margin appeared a small amount necrosis at the 9 times,and the acinar necrosis foci could be seen in large range an the 12 times (histopathological score 8.20 ±0.84),the microscope exhibited altered necrotizing pancreatitis.Conclnsion Conventional caerulein doses (50 μg/kg) may induce acute edematous pancreatitis by 6 times of intraperitoneal injection in mice,and 12 times may induce acute necrotizing pancreatitis.
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