食管癌患者手术前后血清p53抗体动态变化的临床研究  

The clinical study of pre-and post-operative sequential changes of serum p53 antibodies in patients with esophageal squamous cell cancer

作　　者：杨海平[1] 吴骏[1] 刘俊峰[2] 刘紫强[3] 李卫强[1] 郝懿[1] 

机构地区：[1]首都医科大学附属北京潞河医院胸心外科,101100 [2] 河北医科大学第四医院胸三科 [3]首都医科大学附属北京潞河医院检验科,101100

出　　处：《中华实验外科杂志》2015年第4期890-892,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 探讨食管鳞癌患者手术前后血清p53抗体动态变化规律及其与临床病理特征之间的关系.方法 选取因食管鳞癌行食管癌根治术患者98例,其中男68例,女30例,对照组为门诊健康体检者30例,采用酶联免疫吸附法（ELISA）分别于术前1d、术后第7、30、90、180天检测食管癌患者血清p53抗体,并与患者临床病理特征相结合进行研究.结果 （1）食管癌患者血清p53抗体浓度[（338.96±104.14） ng/L比（242.30±39.79） ng/L]和阳性率（37.8％比0）均明显高于对照组（P＜0.05）.（2）食管癌患者血清p53抗体阳性率与患者性别、年龄、肿瘤体积无关（P＞0.05）,而与患者吸烟量、肿瘤组织分化程度、TNM分期呈正相关（P＜0.05）.（3）在行食管癌根治性切除术后,食管癌患者血清p53抗体浓度呈下降趋势,术后第30天基本降至正常水平.结论 （1）血清p53抗体可以作为食管鳞癌诊断和判断其预后的潜在标志物.（2）监测食管鳞癌患者术后的血清p53抗体水平对早期发现其复发或转移有重要的临床参考价值.Objective To explore the relationship between serum p53 antibodies and clinicopathological characteristics in patients with esophageal squamous cell carcinoma （ESCC） and to investigate sequential changing regularity of serum p53 antibodies before and after surgical resection.Methods The eligible ESCC cases of this study were consecutively recruited between October 2010 and February 2012 from Luhe Hospital.There were 68 males and 30 females.The serum p53 antibody concentration was detected in ESCC patients and in 30 healthy adults as controls by enzyme linked immunosorbent assay （ELISA）.The blood samples were collected on the day before operation and on the 7th,30th,90th and 180th days postoperatively.Results （1） The concentration [（338.96 ＋ 104.14） ng/L vs.（242.30 ±39.79） ng/L] and positive rate （37.8% vs.0） of serum p53 antibodies in patients with ESCC were significantly higher than normal individuals （P 〈 0.05）.（2） The positive rate of pre-operative serum p53 antibodies in patients with ESCC positively correlated with the pieces of consumed cigarette,cell differentiation and TNM （P 〈 0.05）.（3） The level of serum p53 antibodies decreased gradually in patients with ESCC underwent radical resection of the cancer,and completely returned to the normal level around the 30th day after operation.Conclusion （1） Serum p53 antibody can be used as a potential marker for early detection of ESCC and predication of prognosis.（2） It has important clinical reference value for detecting the early recurrence or metastasis to monitor serum p53 antibodies level on postoperative patients with ESCC.

关 键 词：食管鳞状细胞癌 P53基因 酶联免疫吸附试验 血清P53抗体 

分 类 号：R735.1[医药卫生—肿瘤]