3株拮抗内生放线菌的鉴定及其抑菌活性初步研究  

The Identification of Three Antagonistic Endophytic Actinomycete and Their Antimicrobial Activities

作　　者：熊瑶瑶[1] 敖武[1,3] 李怡[1] 何晓峰[2] 张志斌[1] 朱笃[1,2] 

机构地区：[1]江西师范大学生命科学学院,江西省亚热带植物资源保护与利用重点实验室,江西南昌330022 [2]江西科技师范大学生命科学学院,江西省生物加工过程重点实验室,江西南昌330013 [3]江西博雅生物制药股份有限公司,江西抚州344000

出　　处：《江西师范大学学报（自然科学版）》2015年第2期171-176,共6页Journal of Jiangxi Normal University(Natural Science Edition)

基　　金：国家自然科学基金(31260137);国家“十二五”重大科技支撑课题(2011BAC13B04);江西省青年科学基金(20132BAB214006);江西省教育厅科研课题(GJJ14250)资助项目

摘　　要：对分离自江西东乡野生稻根部的3株内生放线菌进行鉴定和抑菌活性研究,根据菌株的形态与培养特征、生理生化特性及16S rRNA基因序列系统发育分析,将菌株FRo1、FRo2和FRo3分别鉴定为委内瑞拉链霉菌、娄彻氏链霉菌和肉质链霉菌.采用管碟法和菌丝生长速率法分析菌株拮抗病原细菌与病原真菌的活性.结果表明:菌株FRo1发酵液对所有测试细菌均有抑制作用,显示较广谱抑细菌活性;菌株FRo2显示出较强抑制金黄色葡萄球菌和7种病原真菌的活性,其中对金黄色葡萄球菌抑菌直径为22.33 mm,对小麦赤霉、水稻纹枯病菌、车前草核盘菌及胶胞炭疽菌的抑制率分别为70%、45%、58%和65%;而菌株FRo3发酵液对金黄色葡萄球菌、枯草芽孢杆菌及伤寒杆菌表现出不同程度的抑制,除车前草核盘菌外对所测试的病原真菌均有抑制活性,但抑制率均低于50%,这些菌株抑菌效果良好,显示出较好的生防潜力.Antagonistic strains FRo1,FRo2 and FRo3 were identified,and their antimicrobial activity were assessed,which obtained from roots of Dongxiang wild rice（ Oryza rufipogon）. Strains FRo1,FRo2 and FRo3 was identified as Streptomyces venezuelae,Streptomyces rochei and Streptomyces carnosus by morphological and cultural traits,physiobiochemical characteristics and 16S rRNA gene sequence analysis. The inhibition effects of FRo1,FRo2 and FRo3 against pathogenic bacteria and pathogenic fungi in vitro were evaluated by agar disc diffusion method and growth rate method. The FRo1 fermentation broth showed a wide pectrum of inhibitory activity against tested bacteria. FRo2 fermentation broth had obviously inhibitory activity against staphylococcus aureus and seven pathogenic fungi. The inhibition zone of FRo2 against staphylococcus aureus was about 22. 33mm,and the inhibition ratios of FRo2 against pathogenic fungi Fusarium graminearumt,Rhizoctonia solani,Sclerotinia scleroliorum and Colletotrichum gloeosporioides were 70% 、45% 、58% and 65%,respectively. While FRo3 had different inhibition effects on Staphylococcus aureus,Bacillus subtilis and Typhoid bacillus,and the inhibition activity against tested pathogenic fungi except Sclerotinia scleroliorum was no more than 50%. The strains had better antibacterial effect and could be used for biocontrol.

关 键 词：内生放线菌 链霉菌属 多相分类 抑菌活性 

分 类 号：S718.81[农业科学—林学]